Physiological function of PACAP in the teleost fish

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 16570051
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: University of Toyama
• Principal Investigator: MATSUDA Kouhei University of Toyama, Graduate School of Science and Engineering, Associate Professor, 大学院・理工学研究部, 助教授 (60222303)
• Project Period (FY): 2004 – 2005
• Keywords: Teleost / Neuropeptides / PACAP / Brain / Pituitary / feeding behavior / anorexigenic activity / 摂食制御作用

Research Abstract

The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) could play many roles in mammals. In this study, we used immunochemical techniques to examine the histological and quantitative distribution of PACAP in the central nervous system (CNS) of teleost fish. In addition, high performance liquid chromatographic (HPLC) analysis was performed to characterize the form of PACAP present, while the relationship between PACAP and adenohypophysial hormones was also determined immunohistochemically. We also used enzyme immunoassay to determine the quantity of PACAP in the fish brain, in conjunction with the body mass and gonad somatic index (GSI) of fish. We finally investigated the effects of intracerebroventricular (ICV) and intraperitoneal (IP) administration of synthetic PACAP and related peptides on food intake in the fish. In adult fish, PACAP-like immunoreactive (PACAP-LI) nerve fibers and endings were identified in both the neurohypophysis and adenohypophysis in close proximity to pituitary cells containing immunoreactive hormones such as prolactin, somatolactin, the N-terminal peptide of proopiomelanocortin, and N-acetyl endorphin. PACAP-LI nerve fibers were also identified close to immunoreactive GH cells in the pituitary of young fish. The concentration of immunoreactive PACAP in whole brain ranged from 100 to 800 pmol/g wet weight, in fish with weighing 70-480 g. A negative correlation was found between the concentration of immunoreactive PACAP in the whole brain and body weight, but there was no relation between the former and GSI. In reverse-phase HPLC experiments, the predominant form of immunoreactive PACAP eluted closely with synthetic fish PACAP38, while PACAP27-like immunoreactivity was negligible. Cumulative food intake was significantly decreased by ICV injection of PACAP during a 60-min observation period after treatment. IP administration of PACAP also induced a significant decrease in food intake during a 60-min observation period after treatment. Semiquantitative analysis revealed that the expression of mRNAs for PACAP and the PAC_1 receptor was significantly increased by excessive feeding (daily food supply at【greater than or equal】5% of the body weight [BW]) for 7 days. ICV administration of PACAP induced a significant decrease in locomotor activity during the 60-min post-treatment observation period. Semiquantitative analysis revealed that the expression of CRH mRNA was significantly increased by excessive feeding for 7 days. ICV administration of PACAP at a dose sufficient to suppress food intake induced a significant increase in the expression of CRH mRNA. We also examined the effect of α-helical CRH_<9-41>, a CRH antagonist, on the anorexigenic action of PACAP in the goldfish. The inhibitory effect of PACAP was completely suppressed by treatment with α-helical CRH_<9-41>. These results indicate that PACAP also plays many roles in fish.

Research Products

• [Journal Article] Regulation of food intake by acyl and des-acyl ghrelins in the goldfish. (2006)
  • Author(s): Matsuda K., Miura T., Kaiya H., Maruyama K., Shimakura S.I., Uchiyama M., Kangawa K., Shioda S.
  • Journal Title: Peptides (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Pituitary adenylate cyclase-activating polypeptide (PACAP) decreases neuronal cell death after focal ischemia associated with interleukin-6 (IL-6) signaling. (2006)
  • Author(s): Ohtaki H., Nakamachi T., Dohi K., Aizawa Y.Takaki A., Hodoyama K., Yofu S., hashimoto H, Shintani N., Baba A., Kopf M., Iwakura Y., Matsuda K., Arimura A., Shioda S.
  • Journal Title: Proc.Natl.Acad.Sci. 103 Pages: 7488-7493
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Central administration of melanin-concentrating hormone (MCH) suppresses food intake, but not locomotor activity, in the goldfish, Carassius auratus. (2006)
  • Author(s): Matsuda K., Shimakura S.I., Maruyama K., Miura T., Uchiyama M., Kawauchi H., Shioda S., Takahashi A.
  • Journal Title: Neurosci Lett 399 Pages: 259-263
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of osmotic stress on expression of a putative facilitative urea transporter in the kidney and urinary bladder of the marine toad, Bufo marinus. (2006)
  • Author(s): Konno N., Hyodo S., Matsuda K., Uchiyama M.
  • Journal Title: J.Exp.Biol. 209 Pages: 1207-1216
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Relationship between anorexigenic action of pituitary adenylate cyclase-activating polypeptide (PACAP) and that of corticotropin-releasing hormone (CRH) in the goldfish, Carassius auratus. (2006)
  • Author(s): Maruyama K., Miura T., Uchiyama M., Shioda S., Matsuda.K.
  • Journal Title: Peptides (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Regulation by orexin of feeding behaviour and locomotor activity in the goldfish. (2006)
  • Author(s): Nakamachi T., Matsuda K., Maruyama K., Miura T., Uchiyama M., Funahashi H., Sakurai T., Shioda S.
  • Journal Title: J.Neuroscience Lett. 18 Pages: 9-13
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Stimulatory effect of n-octanoylated ghrelin on locomotor activity in the goldfish, Carassius auratus. (2006)
  • Author(s): Matsuda K., Miura T., Kaiya H., Maruyama K., Uchiyama M., Kangawa K., Shioda S.
  • Journal Title: Peptides (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Atrial natriuretic peptide and cGMP active sodium transport through PKA-dependent pathway in the urinary bladder of the Japanese tree frog. (2006)
  • Author(s): Yamada T., Matsuda K., Uchiyama M.
  • Journal Title: J.Comp.Physiol. B176 Pages: 203-212
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effects of pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) on food intake and locomotor activity in the goldfish, Carassius auratus. (2006)
  • Author(s): Matsuda K., Maruyama K., Nakamachi T., Miura T., Shioda S.
  • Journal Title: Ann.N.Y.Acad.Sci. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Regulation of food intake by acyl and des-acyl ghrelins in the goldfish. (2006)
  • Author(s): Matsuda, K., Miura, T., Kaiya, H., Maruyama, K., Shimakura, S.I., Uchiyama, M., Kangawa, K., Shioda, S.
  • Journal Title: Peptides (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pituitary adenylate cyclase-activating polypeptide (PACAP) decreases neuronal cell death after focal ischemia associated with inteleukin-6 (IL-6) signaling. (2006)
  • Author(s): Ohtaki, H., Nakamachi, T., Dohi, K., Aizawa, Y., Takaki, A., Hodoyama, K., Yofu, S., Hashimoto, H., Shintani, N., Baba, A., Kopf, M., Iwakura, Y., Matsuda, K., Arimura, A., Shioda, S.
  • Journal Title: Proc.Natl.Acad.Sci.USA 103 Pages: 7488-7493
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Central administration of melanin-concentrating hormone (MCH) suppresses food intake, but not locomotor activity, in the goldfish, Carassius auratus. (2006)
  • Author(s): Matsuda, K., Shimakura, S.I., Maruyama, K., Miura, T., Uchiyama, M., Kawauchi, H., Shioda, S., Takahashi, A.
  • Journal Title: Neurosci.Lett. 399 Pages: 259-263
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of osmotic stress on expression of a putative facilitative urea transporter in the kidney and urinary bladder of the marine toad, Bufo marinus. (2006)
  • Author(s): Konno, N., Hyodo, S., Matsuda, K., Uchiyama, M.
  • Journal Title: J.Exp.Biol. 209 Pages: 1207-1216
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Relationship between anorexigenic action of pituitary adenylate cyclase-activating polypeptide (PACAP) and that of corticotropin-releasing hormone (CRH) in the goldfish, Carassius, auratus. (2006)
  • Author(s): Maruyama, K., Miura, T., Uchiyama, M., Shioda, S., Matsuda, K.
  • Journal Title: Peptides (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regulation by orexin of feeding behaviour and locomotor activity in the goldfish. (2006)
  • Author(s): Nakamachi, T., Matsuda, K., Maruyama, K., Miura, T., Uchiyama, M., Funahashi, H., Sakurai, T., Shioda, S.
  • Journal Title: J.Neuroendocrinol. 18 Pages: 290-297
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Stimulatory effect of n-octanoylated ghrelin on locomotor activity in the goldfish, Carassius auratus. (2006)
  • Author(s): Matsuda, K., Miura, T., Kaiya, H., Maruyama, K., Uchiyama, M., Kangawa, K., Shioda, S.
  • Journal Title: Peptides (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Atrial natriuretic peptide and cGMP active sodium transport through PKA-dependent pathway in the urinary bladder of the Japanese tree frog. (2006)
  • Author(s): Yamada, T., Matsuda, K., Uchiyama, M.
  • Journal Title: J.Comp.Physiol.B 176 Pages: 203-212
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) on food intake and locomotor activity in the goldfish, Carassius auratus. (2006)
  • Author(s): Matsuda, K., Maruyama, K., Nakamachi T., Miura, T., Shioda, S.
  • Journal Title: Ann.N.Y.Acad.Sci. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Seasonal variations in plasma concentrations of sex steroid hormones and vitellogenin in wild male Japanese Dace (Tribolodon hakonensis) collected from different sites of the Jinzu river basin. (2005)
  • Author(s): Ma Y-X., Matsuda K., Uchiyama M.
  • Journal Title: Zool.Sci. 22 Pages: 861-868
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Anorexigenic action of pituitary adenylate cyclase-activating polypeptide (PACAP) in the goldfish : feeding-induced changes in the expression of mRNAs for PACAP and its receptors in the brain, and locomotor response to central injection. (2005)
  • Author(s): Matsuda K., Maruyama K., Miura T., Uchiyama M., Shioda S.
  • Journal Title: Neurosci.Lett. 386 Pages: 9-13
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Inhibitory effects of pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) on food intake in the goldfish, Carassius auratus. (2005)
  • Author(s): Matsuda K., Maruyama K., Nakamachi T., Miura T., Uchiyama M., Shioda S.
  • Journal Title: Peptides 26 Pages: 1611-1616
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immunohistochemical observation of pituitary adenylate cyclase-activating polypeptide (PACAP) and adenohypophysial hormones in the pituitary of a teleost, Uranoscopus japonicus. (2005)
  • Author(s): Matsuda K., Nagano Y., Uchiyama M., Takahashi A., Kawauchi H.
  • Journal Title: Zool.Sci. 22 Pages: 71-76
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Pituitary adenylate cyclse-activating polypeptide (PACAP)-like immunoreactivity in the brain of a teleost, Uranoscopus japonicus : immunohistochemical relationship between PACAP and adenohypophysial hormones. (2005)
  • Author(s): Matsuda K., Nagano Y., Uchiyama.M., Onoue S., Takahashi A., Kawauchi H., Shioda S.
  • Journal Title: Regul.Pept. 126 Pages: 129-136
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Orexin-1 receptor expression after global ischemia in mice. (2005)
  • Author(s): Nakamachi T., Ohtaki H., Yin L., Dohi K., Endo S., Kudo Y., Funahashi H., Matsuda K., Shioda S.
  • Journal Title: Regul.Pept. 126 Pages: 49-54
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Plasma aldosterone, angiotensin II and arginine vasotocin concentrations in the toad, Bufo marinus, following osmotic treatments. (2005)
  • Author(s): Konno N., Hyodo S., Takei Y., Matsuda K., Uchiyama M.
  • Journal Title: Gen.Comp.Endocrinol. 140 Pages: 86-93
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Seasonal variations in plasma concentrations of sex steroid hormones and vitellogenin in wild male Japanese Dace (Tribolodon hakonensis) collected from different sites of the Jinzu river basin. (2005)
  • Author(s): Ma, Y-X., Matsuda, K., Uchiyama, M.
  • Journal Title: Zool.Sci. 22 Pages: 861-868
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Anorexigenic action of pituitary adenylate cyclase-activating polypeptide (PACAP) in the goldfish : feeding-induced changes in the expression of mRNAs for PACAP and its receptors in the brain, and locomotor response to central injection. (2005)
  • Author(s): Matsuda, K., Maruyama, K., Miura, T., Uchiyama, M., Shioda, S.
  • Journal Title: Neurosci.Lett. 386 Pages: 9-13
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibitory effects of pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) on food intake in the goldfish, Carassius auratus. (2005)
  • Author(s): Matsuda, K., Marayama, K., Nakamachi, T., Miura, T., Uchiyama, M., Shioda, S.
  • Journal Title: Peptides 26 Pages: 1611-1616
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immunohistochemical observation of pituitary adenylate cyclase-activating polypeptide (PACAP) and adenohypophysial hormones in the pituitary of a teleost, Uranoscopus japonicus. (2005)
  • Author(s): Matsuda, K., Nagano, Y., Uchiyama, M., Takahashi, A., Kawauchi, H.
  • Journal Title: Zool.Sci. 22 Pages: 71-76
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pituitary adenylate cycles-activating polypeptide (PACAP)-like immunoreactivity in the brain of a teleost, Uranoscopus japonicus : immunohistochemial relationship between PACAP and adenohypophysial hormones. (2005)
  • Author(s): Matsuda, K., Nagano, Y., Uchiyama, M., Onoue, S., Takahashi, A., Kawauchi, H., Shioda, S.
  • Journal Title: Regul.Pept. 126 Pages: 129-136
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Orexin-1 receptor expression after global ischemia in mice. (2005)
  • Author(s): Nakamachi, T., Ohtaki, H., Yin, L., Dohi, K., Endo, S., Kudo, Y., Funahashi, H., Matsuda, K., Shioda, S.
  • Journal Title: Regul.Pept. 126 Pages: 49-54
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Plasma aldosterone, angiotensin II and arginine vasotocin concentrations in the toad, Bufo marinus, following osmotic treatments. (2005)
  • Author(s): Konno, N., Hyodo, S., Takei, Y., Matsuda, K., Uchiyama, M.
  • Journal Title: Gen.Comp.Endocrinol. 140 Pages: 86-93
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Distribution of PACAP and its binding sites in the brain of a teleost, the stargazer. (2004)
  • Author(s): Matsuda K., Nagano Y., Uchiyama M., Onoue S., Kashimoto K., Takahashi A., Kawauchi H.
  • Journal Title: Trends in Comparative Endocrinology Pages: 282-285
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ghrelin stimulates food intake in the goldfish. (2004)
  • Author(s): Matsuda K., Miura T., Hagiwara T., Uchiyama M., Kaiya H., Kangawa K.
  • Journal Title: Trends in Comparative Endocrinology Pages: 286-288
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effects of atrial natriuretic peptide and cGMP on epithelial Na transport in frog urinary bladder. (2004)
  • Author(s): Yamada T., Nishio T., Matsuda K., Narita K., Uchiyama M.
  • Journal Title: Trends in Comparative Endocrinology Pages: 350-353
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Functional characrerization of Cl^- transport activated by forskolin with the short-circuiting technique in the skin of two anuran species (Hyla japonica and Rana nigromaculata). (2004)
  • Author(s): Nishio T., Yamada T., Sano Y., Matsuda K., Uchiyama M.
  • Journal Title: Trends in Comparative Endocrinology Pages: 366-368
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Distribution of PACAP and its binding sites in the brain of a teleost, the stargazer. (2004)
  • Author(s): Matsuda, K., Nagano, Y., Uchiyama, M., Onoue, S., Kashimoto, K., Takahashi, A., Kawauchi, H.
  • Journal Title: Trends in Comparative Endocrinology Pages: 282-285
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ghrelin stimulates food intake in the goldfish. (2004)
  • Author(s): Matsuda, K., Miura, T., Hagiwara, T., Uchiyama, M., Kaiya, H., Kangawa, K.
  • Journal Title: Trends in Comparative Endocrinology Pages: 286-288
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of atrial natriuretic peptide and cGMP on epithelial Na transport in frog urinary bladder. (2004)
  • Author(s): Yamada, T., Nishio, T., Matsuda, K., Narita, K., Uchiyama, M.
  • Journal Title: Trends in Comparative Endocrinology Pages: 350-353
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional characrerization of Cl- transport activated by forskolin with the short-circuiting technique in the skin of two anuran species (Hyla japonica and Rana nigromaculata). (2004)
  • Author(s): Nishio, T., Yamada, T., Sano, Y., Matsuda, K., Uchiyama, M.
  • Journal Title: Trends in Comparative Endocrinology Pages: 366-368
  • Description: 「研究成果報告書概要(欧文)」より