2005 Fiscal Year Final Research Report Summary
Regulation of matrix metalloproteinase activity via sugar chain
| Project/Area Number |
16570117
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kagawa University |
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Principal Investigator |
NISHI Nozomu Kagawa University, Faculty of Medicine, Department of Endocrinology, Research Associate, 医学部, 助手 (10145047)
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| Project Period (FY) |
2004 – 2005
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| Keywords | galectin / matrix metalloproteinase / oligosaccharide / lectin / protease / glycoprotein / neutrophil / inflammation |
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| Research Abstract |
1.Oligosaccharide structures of pro-MMP-9 recognized by galectin-8 : It has been reported that pro-MMP-9 has three N-linked oligosaccharides and many O-linked oligosaccharides. We have tested the effect of removal of the N-linked oligosaccharides on the interaction with galectin-8.pro-MMP-9 deficient in N-linked oligosaccharides retained the ability to interact with galectin-8 suggesting that O-linked oligosaccharides are important in galectin-8-mediated activation of pro-MMP-9. However, removal of O-linked oligosaccharides of pro-MMP-9 with a combination of O-glycanase, sialidase A and β(1-4)galactosidase did not abolish the ability to interact with galectin-8. These results suggest that galectin-8 can bind to both N- and O-linked oligosaccharides of proMMP-9.
2.Mechanism for acceleration of pro-MMP-9 activation by galectin-8 : We have already reported that N-terminal carbohydrate recognition domain (N-CRD) of galectin-8 has high affinity for pro-MMP-9 and that inactivation of either N- or C-CRD by site-directed mutagenesis resulted in loss of the activity (acceleration of pro-MMP-9 activation by MMP-3). MMP-3 binds to both N- and C-CRD of galectin-8 with similar affinities. In addition, we found that tag-free galectin-8 exhibits lower activity than that of GST-galectin-8. Based on these results, we speculate that galectin-8 increases the probability of interaction between pro-MMP-9 and MMP-3 in dilute solutions by binding both components (pro-MMP-9 at N-CRD and MMP-3 at C-CRD). The effect of GST-moiety can be explained by stable dimer formation of GST-galectin-8 but not tag-free galectin-8, which further increases the probability of the interaction.
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[Journal Article] Xenopus galectin-VIIa binds N-glycans of members of the cortical granule lectin family (xCGL and xCGL2).2005
Author(s)
Shoji, H., Ikenaka, K., Nakakita, S., Hayama, K., Hirabayashi, J., Arata, Y., Kasai, K., Nishi, N., Nakamura, T.
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Journal Title
Glycobiology 15(7)
Pages: 709-720
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Development of highly stable galectins : Truncation of the linker peptide confers protease-resistance on tandem-repeat type galectins.2005
Author(s)
Nishi, N., Itoh, A., Fujiyama, A., Yoshida, N., Araya, S., Hirashima, M., Shoji, H., Nakamura, T.
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Journal Title
FEBS Lett. 579(10)
Pages: 2058-2064
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Galectin-9 as a prognostic factor with antimetastatic potential in breast cancer.2005
Author(s)
Irie, A., Yamauchi, A., Kontani, K., Kihara, M., Liu, D., Shirato, Y., Seki, M., Nishi, N., Nakamura, T., Yokomise, H., Hirashima, M.
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Journal Title
Clin.Cancer Res. 11(8)
Pages: 2962-2968
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In nasopharyngeal carcinoma cells, Epstein-Barr virus LMP1 interacts with galectin 9 in membrane raft elements resistant to simvastatin.2005
Author(s)
Pioche-Durieu, C., Keryer, C., Souquere, S., Bosq, J., Faigle, W., Loew, D., Hirashima, M., Nishi, N., Middeldorp, J., Busson, P.
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Journal Title
J.Virol. 79(21)
Pages: 13326-13337
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Retinoic acid-inducible gene-1 is induced by interleukin-1 beta in cultured human gingival fibroblasts.2005
Author(s)
Sakaki, H., Imaizumi, T., Matsumiya, T., Kusumi, A., Nakagawa, H., Kubota, K., Nishi, N., Nakamura, T., Hirashima, M., Satoh, K., Kimura, H.
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Journal Title
Oral.Microbiol.Immunol. 20(1)
Pages: 47-50
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Galectin-9 in physiological and pathological conditions.2004
Author(s)
Hirashima, M., Kashio, Y., Nishi, N., Yamauchi, A., Imaizumi, T., Kageshita, T., Saita, N., Nakamura, T.
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Journal Title
Glycoconj.J. 19(7-9)
Pages: 593-600
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Double-stranded RNA enhances the expression of galectin-9 in vascular endothelial cells.2004
Author(s)
Ishikawa, A., Imaizumi, T., Yoshida, H., Nishi, N., Nakamura, T., Hirashima, M., Satoh, K.
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Journal Title
Immunol.Cell Biol. 82(4)
Pages: 410-414
Description
「研究成果報告書概要(欧文)」より
