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2006 Fiscal Year Final Research Report Summary

Phosphorylation of heat shock transcription factor in the stress response

Research Project

Project/Area Number 16570142
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionKanazawa University

Principal Investigator

SAKURAI Hiroshi  Kanazawa University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (00225848)

Project Period (FY) 2004 – 2006
Keywordsstress response / heat shock transcription factor / phosphorylation / yeast
Research Abstract

All organisms respond to elevated temperatures by changing transcription programs and expressing a set of proteins called heat shock proteins (HSPs). In eukaryotes, heat sock transcription factor (HSF) activates transcription of HSP genes. The target genes of HSF contain a cis-acting sequence designated the heat shock element. The baker's yeast, Saccharomyces cerevisiae, has one HSF encoded by the HSF1 locus. Under heat shock condition, Hsfl protein is extensively phosphorylated and acquires a stronger activating ability, although detailed regulatory mechanisms remain unknown. In this project, I focused on mechanisms of Hsfl phosphorylation and dephosphorylation' and 'roles of Hsfl in the response to various stresses'.
By using DNA microarray analysis, we have shown that yeast Hsfl is necessary for heat-induced transcription of〜70 genes and that target genes encode molecular chaperones and proteins involved in a broad range biological functions. We have characterized multicopy suppressor genes of a temperature-sensitive hsfl mutation and found that Hsfl in concert with a protein kinase Pkc1 regulates cell wall remodeling in response to heat shock. Heat-induced hyperphosphorylation of Hsfl was required for transcriptional activation of approximately half of Hsfl target genes. This requirement was related to cooperative interactions among Hsfl trimers bound to the HSE. Consistent with this, oligomerization of Hsfl was prerequisite for stress-induced hyperphosphorylation of Hsfl. We have also shown that human HSF1 recognizes HSEs in a slightly different way than yeast Hsfl.
Taken together, these results suggest that the architecture of the HSE is an important determinant for HSF-HSE interactions

  • Research Products

    (18 results)

All 2007 2006 2005 2004

All Journal Article (18 results)

  • [Journal Article] Interaction between heat shock transcription factors (HSFs) and divergent binding sequences.2007

    • Author(s)
      Sakurai, H., Takemori, Y.
    • Journal Title

      Journal of Biological Chemistry 282・18

      Pages: 13334-13341

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Different mechanisms are involved in the transcriptional activation by heat shock transcription factor through different types of heat shock elements.2007

    • Author(s)
      Hashikawa, N. et al.
    • Journal Title

      Journal of Biological Chemistry 282・14

      Pages: 10333-10340

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Interaction between heat shock transcription factors (HSFs) and divergent binding sequences2007

    • Author(s)
      Hiroshi Sakurai, Yukiko Takemori
    • Journal Title

      Journal of Biological Chemistry 282・18

      Pages: 13334-13341

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Different mechanisms are involved in the transcriptional activation by heat shock transcription factor through different types of heat shock elements2007

    • Author(s)
      Naoya Hashikawa, Noritaka, Yamamoto, Hiroshi Sakurai
    • Journal Title

      Journal of Biological Chemistry 282・14

      Pages: 10333-10340

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The DNA-binding domain of yeast Hsf1 regulates both DNA binding and transcriptional activities.2006

    • Author(s)
      Yamamoto, A., Sakurai H.
    • Journal Title

      Biochemical and Biophysical Research Communications 346・4

      Pages: 1324-1329

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mutated yeast heat shock transcription factor activates transcription independently of hyperphosphorylation.2006

    • Author(s)
      Hashikawa, N. et al.
    • Journal Title

      Journal of Biological Chemistry 281・7

      Pages: 3936-3942

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stress-induced transcription of the endoplasmic reticulum oxidoreductin gene ERO1 in the yeast Saccharomyces cerevisiae2006

    • Author(s)
      Takemori, Y. et al.
    • Journal Title

      Molecular Genetics and Genomics 275・1

      Pages: 89-96

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The DNA-binding domain of yeast Hsfl regulates both DNA binding and transcriptional activities2006

    • Author(s)
      Ayako Yamamoto, Hiroshi Sakurai
    • Journal Title

      Biochemical and Biophysical Research Communications 346・4

      Pages: 1324-1329

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mutated yeast heat shock transcription factor activates transcription independently of hyperphosphorylation2006

    • Author(s)
      Naoya Hashikawa, Yu Mizukami, Hiromi Imazu, Hiroshi Sakurai
    • Journal Title

      Journal of Biological Chemistry 281・7

      Pages: 3936-3942

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Stress-induced transcription of the endoplasmic reticulum oxidoreductin gene ERO1 in the yeast Saccharomyces cerevisiae2006

    • Author(s)
      Yukiko Takemori, Ayako Sakaguchi, Saori, Matsuda, Yu Mizukami, Hiroshi Sakurai
    • Journal Title

      Molecular Genetics and Genomics 275・1

      Pages: 89-96

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 熱ショック転写因子によるストレス応答の制御2005

    • Author(s)
      桜井 博
    • Journal Title

      生化学 77・4

      Pages: 347-350

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock2005

    • Author(s)
      Imazu, H. et al.
    • Journal Title

      Eukaryotic Cell 4・6

      Pages: 1050-1056

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of a novel class of target genes and a novel type of binding sequence of heat shock transcription factor in Saccharomyces cerevisiae2005

    • Author(s)
      Yamamoto, A. et al.
    • Journal Title

      Journal of Biological Chemistry 280・12

      Pages: 11911-11919

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of stress response by heat shock transcription factor2005

    • Author(s)
      Hiroshi Sakurai
    • Journal Title

      Seikagaku 77・4

      Pages: 347-350

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock2005

    • Author(s)
      Hiromi Imazu, Hiroshi Sakurai
    • Journal Title

      Eukaryotic Cell 4・6

      Pages: 1050-1056

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of a novel class of target genes and a novel type of binding sequence of heat shock transcription factor in Saccharomyces cerevisiae2005

    • Author(s)
      Ayako Yamamoto, Yu Mizukami, Hiroshi Sakurai
    • Journal Title

      Journal of Biological Chemistry 280・12

      Pages: 11911-11919

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Phosphorylation of the yeast heat shock transcription factor is implicated in gene-specific activation dependent on the architecture of the heat shock element2004

    • Author(s)
      Hashikawa, N., Sakurai, H.
    • Journal Title

      Molecular and Cellular Biology 24・9

      Pages: 3648-3659

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Phosphorylation of the yeast heat shock transcription factor is implicated in gene-specific activation dependent on the architecture of the heat shock element2004

    • Author(s)
      Naoya Hashikawa, Hiroshi Sakurai
    • Journal Title

      Molecular and Cellular Biology 24・9

      Pages: 3648-3659

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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