2005 Fiscal Year Final Research Report Summary
A Study of Barrier-to-Autointegration Factor (BAF) function for nuclear and chromosomal structures in Drosophila.
| Project/Area Number |
16570155
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Niigata University |
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Principal Investigator |
FURUKAWA Kazuhiro Niigata University, Institute of Science and Technology, Associate Professor, 自然科学系, 助教授 (40229109)
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| Project Period (FY) |
2004 – 2005
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| Keywords | BAF / Nuclear envelope / lamin / apoptosis |
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| Research Abstract |
We analyzed the imaginal disc development in Drosophila baf null mutants, and found several small degenerative tissues around the CNS at the young larvae. Immunohistochemical analyses showed that diffusible lamin distribution and drICE activation in the imaginal discs, but not in CNS. Thus the imaginal discs selectively enter apoptosis during loss of BAF at the early larval development. We further investigated the fate of BAF during apoptosis of imaginal disc and CNS by a heat-inducible hid transgene. During this apoptotic execution, the rapidly loss of nuclear BAF was observed at the early stage similar as lamin Dm_0 degradation. Further, the loss of DNA binding forms of BAF preceded that of non-DNA binding forms of BAF. In addition, TUNEL labeling indicated that the DNA binding forms of BAF disappeared in nuclei before obvious DNA fragmentation was detected. BAF loss was not detected before drICE activation and was inhibited by Ac-DEVD-CHO caspase inhibitors, thus disappearance of these BAF is dependent on a caspase function. Together with the nuclear phenotypes of imaginal disc and CNS tissues of baf null mutants, we suggest that the early target of chromosomal BAF in caspase cascade is involved in changing of the early nuclear morphological changes during apoptosis.
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[Journal Article] Dissociation of emerin from barrier-to-autointegration factor is regulated by mitotic phosphorylation of emerin in Xenopus egg cell-free system.2005
Author(s)
Hiranoa, Y., Segawaa, M., Honmaa, S., Ouchib, F.S., Yamakawab, Y., Takeyasu, K., Furukawaa, K., Horigomea, T.
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Journal Title
J.Biol.Chem. 280
Pages: 39925-39933
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Null mutants of Drosophila B-type lamin Dm0 show aberrant tissue differentiation rather than obvious nuclear shape distortion of specific defects during cell proliferation.2005
Author(s)
Osouda, S., Nakamura Y., de Saint Phalle, B.McConnell, B., Horigome, T., Sugiyama, S., Fisher, P.A., Furukawa, K.
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Journal Title
Dev.Biol. 284
Pages: 219-232
Description
「研究成果報告書概要(欧文)」より
