2005 Fiscal Year Final Research Report Summary
Physiological and Molecular Biological Analyses of seed Germination Ecology in Echinochloa crus-galli (L.) Beauv., a weed in flooded rice
| Project/Area Number |
16580011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Crop science/Weed science
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMASUE Yuji Kyoto University, Graduate School of Agri., Professor, 農学研究科, 教授 (60093332)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Echinocloa plants / Seed ecology / Anaerobic germination / Flooding-tolerance / Flooding-susceptibility / Alcohol fermentation / Pastuer effcet / ADH inhibitor |
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| Research Abstract |
Flooding-tolerant and -susceptible varieties of Echinochloa crus-galli (L.) Beauv. were used to study (1) physiological and molecular analyses of the flooding tolerant seed germination and (2) metabolic regulation of seed respiration under flooding.
1. Physiological and molecular analyses of flooding-tolerant seed germination
In flooding-tolerant variety, seeds imbibed under N_2 could exhibited large I/N quotient and accelerated glycolysis and concomitantly produced large and equimolar quantities of CO_2 and ethanol, suggesting that the seeds operated the Pasteur effect and could germinate under anaerobic conditions. In contrast, seeds of the flooding-susceptible variety were capable of germinating only under aerobic conditions through the conventional aerobic respiration, but unable to anaerobically respire to germinate through the alcohol fermentation.
2. Metabolic regulation of seed respiration under flooding
In a rice field where the soil is flooded, seeds of E.oryzicola anaerobically initially respire through alcohol fermentation to germinate and the seedlings switch the fermentation to the conventional aerobic respiration when they reach the water surface. This switching was found to be regulated by an endogenous ADH-inhibiting protein that is induced by oxygen.
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