Investigation of protein secreted from cultured cell line in insect

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 16580037
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: HIRAOKA Tsuyoshi Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Associate Professor, 大学院共生科学技術研究院, 助教授 (10238339)
• Project Period (FY): 2004 – 2006
• Keywords: insect culture cell / functional protein / secreted protein

Research Abstract

Acetylcholinesterase was purified from conditioned medium of insect cell line NIAS-AeA1-2. SDS-PAGE was suggested that molecular weight was 67kDa, and gel-filtrated chromatography suggested this protein was dimmer in conditioned medium.
Growth blocking peptide, an insect EGF type growth factor was detected by RT-PCR in SES-NaBr-4 and SES-NaBr-93 cell line.
Trypsin inhibitor was detected in NIAS-AeA1-2, and SES-MaBr-4. I tried to purify this protein but in step of affinity column chromatography, this protein adsorbed tightly to trypsin. This protein still unclear about molecular weight and function.
Dopa-decalboxilase was detected by RT-PCR in SES-NaBr-4 Cell line