• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2006 Fiscal Year Final Research Report Summary

A basic research for applying the functional food components to reduce the risk of allergic diseases

Research Project

Project/Area Number 16580100
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionThe University of Tokushima

Principal Investigator

YAMANISHI Rintaro  The University of Tokushima, Institute of Health Biosciences, associate professor, 大学院ヘルスバイオサイエンス研究部, 助教授 (30253206)

Co-Investigator(Kenkyū-buntansha) BANDO Noriko  The University of Tokushima, Institute of Health Biosciences, Instructor, 大学院ヘルスバイオサイエンス研究部, 教務員 (40116851)
Project Period (FY) 2004 – 2006
Keywordsβ-carotene / murine splenocytes / glutathione / redox / IL-12 / antigen-presentation / RAW264
Research Abstract

In our previous study we found that combinational feeding of beta-carotene and high alpha-tocopherol to BALB/c mice suppressed the production of IgE antibody induced by the specific antigen. To clear the mechanism underlying this suppression, we have carried out several experiments. The production of cytokine in mice that influence the IgE production was investigated in 2004. An experiment of ex vivo antigen-presentation revealed that the production of the antigen inducible IL-12 was augmented in splenocytes from mice fed with beta-carotene and high alpha-tocohperol. Since the effect was lost when BC was replaced with retinylester, the participation' of its redox activity is instead of provitamin-A activity was considered. We found that BC induced the synthesis of glutathione accompanied by the induction of the light chain of gamma-glutamylcysteine synthetase in RAW264, a murine macrophage cultured cell line, while BC oxidizes the cellular membrane in 2005. The synthesis of glutathione led to the cytoplasmic anti-oxidation which influenced the expression of cytokine mRNAs. Although AT inhibited the oxidization of cellular membrane induced by free-radicals, it could not inhibit the oxidization by BC and it affected neither the anti-oxidization of cytoplasm, nor cytokine mRNA production. Based on above results, we intended to clear the mechanism that BC enhances the IL-12 secretion in mice fed with a fixed amount of AT in 2006. As a main result, we found out that intracellular GSH concentration was up-regulated in the splenocytes dose-dependently on BC. There was also a report of correlating the amount of GSH(s) in an antigen presentation cell and IL-12 secretion, together with the above-mentioned research, we conclude that ingestion of BC results in anti-oxidative change in the cytoplasm of the antigen-presenting-cells, which contributes to enhancing IL-12 production suppressing the production of IgE antibody.

  • Research Products

    (2 results)

All 2006

All Journal Article (2 results)

  • [Journal Article] β-Carotene Modulates the Immunological Function of RAW264, a Murine Macrophage Cell Line, by Enhancing the Level of Intracellular Glutathione2006

    • Author(s)
      Tomomi Imamura
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 70・9

      Pages: 2112-2120

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] β-Carotene Modulates the Immunological Function of RAW264, a Murine Macrophage Cell Line, by Enhancing the Level of Intracellular Glutathione2006

    • Author(s)
      Tomomi Imamura
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 7 0-9

      Pages: 2112-2120

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2008-05-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi