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2005 Fiscal Year Final Research Report Summary

Study of immunomodulation on the intestinal immune responses by saccharides

Research Project

Project/Area Number 16580108
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionNihon University

Principal Investigator

HOSONO Akira  Nihon University, College of Bioresource Sciences, Lecturer, 生物資源科学部, 講師 (70328706)

Project Period (FY) 2004 – 2005
Keywordspolysaccharides derived from Bifidobacterium / IgA / Peyer's patch / mesenteric lymph node / lamina propria / cecal follicle / CD11c^+ cells
Research Abstract

Bifidobacterium immunomodulators (BIM) are composed of water-soluble polysaccharides and peptidoglycans, which are expected to modulate immune responses in the host. We demonstrated that oral administration of bacterial polysaccharides, BIM for 7 consecutive days induced the immunoresponses (e.g. cytokine production) on the murine lymphoid tissues (Peyer's patch ; PP, mesenteric lymphoid nodes ; MLN, and spleen ; SPL). This study is to clarify the distribution of BIM in the host gut-associated lymphoid tissues after single-shot administration of BIM orally, and we are investigating the immune responses of lamina propropria (LP) as an effector site for mucosal IgA secretion by oral administration. We observed the distribution of BIM in PP and LP after oral administration. We have shown that the BIM reached PP and small intestinal LP (S-LP) at the passed time of 1 hour after oral administration and the location of DIM distribution observed in PP tissue sections were almost as same as CD11c^+ cells. Then, BIM reached MLN at the passed time of 18 hours after oral administration. These results suggested that the BIM might pass through the villous M-cells directly and might be endcytosed and processed by CD11c^+ cell such as dendritic cells in PP. It was also suggested that the BIM might pass through the tight junctions of intestinal epithelial cells into LP directly, but not through the common mucosal immune system (CMIS), and then the BIM might be endcytosed by CD11^+ cell such as dendritic cell orally at the passed time of 1 hour after single-shot administration. The findings of the cytokines and IgA responses of LPL indicated that BIM might stimulate S-LPL to secrete IgA and cytokines by BIM feeding. In addition, It suggested that BIM could induce different immunoreactions of LPL in the intestinal location between small intestine and large intestine.

  • Research Products

    (9 results)

All 2005 2004

All Journal Article (7 results) Book (2 results)

  • [Journal Article] Oral administration of food antigen induces T cell mediated intestinal inflammation : A model using TCR-transgenic mice.2005

    • Author(s)
      A.Kikuchi, H.Nakajima-Adachi, A.Ebihara, Y.Takahashi, A Hosono, K.Kaminogawa
    • Journal Title

      Biofactors 21(1-4)

      Pages: 159-162

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characteristic immune response in Peyer's patch cells induced by oral administration of Bifidobacterium components.2005

    • Author(s)
      Y.Nakanishi, A.Hosono, Y.Hiramatsu, T.Kimura, R.Nakamura, S.Kaminogawa
    • Journal Title

      Cytotechnology 47(1-3)

      Pages: 69-77

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of sugar cane extract on the modulation of immunity in pigs.2005

    • Author(s)
      D-Y.Lo, T-H.Chen, M-S.Chien, K.Koge, A.Hosono, S.Kaminogawa, W-C.Lee
    • Journal Title

      The Journal of Veterinary Medical Science 67(6)

      Pages: 591-597

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of sugar cane extract on the modulation of immunity in pigs.2005

    • Author(s)
      D.Y.Lo, T.H.Chen, M.S.Chien, K.Koge, A.Hosono, S.Kaminogawa, W.C.Lee
    • Journal Title

      J.Vet.Med.Sci. 67(6)

      Pages: 591-597

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Establishment and analysis of germfree T cell receptor transgenic mice.2004

    • Author(s)
      M.Fujioka, S.Hachimura, A.Hosono, R.Nakamura, K.Hirayama, I.Itoh, S.Kaminogawa
    • Journal Title

      Animal Cell Technology : Basic & Applied Aspects 13

      Pages: 243-247

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Establishment and analysis of germfree T cell receptor transgenic mice.2004

    • Author(s)
      M.Fujioka, S.Hachimura, A.Hosono, R.Nakamura, K.Hirayama, K.Itoh, S.Kaminogawa
    • Journal Title

      Animal Cell Technology : Basic & Applied Aspects 13

      Pages: 243-247

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Oral administration of food antigen induces T cell mediated intestinal inflammation : A model using TCR-transgenic mice.2004

    • Author(s)
      A.Kikuchi, H.Nakajima-Adachi, A.Ebihara, Y Takahashi, A.Hosono, K.Itoh, S.Hachimura, S.Kaminogawa
    • Journal Title

      Biofactors 21(1-4)

      Pages: 159-162

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] III-3-1 腸管免疫細胞分離法 c)粘膜固有層リンパ球の調製法。(上野川修一・清水誠・八村敏志・戸塚護 編著) : 腸管細胞機能実験法2005

    • Author(s)
      細野 朗
    • Total Pages
      8(274)
    • Publisher
      学会出版センター
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] III-13 プロバイオティクス、 III-14プロバイオティクス.(吉川敏一・桜井弘 編) : サプリメントデータブック2005

    • Author(s)
      細野 朗, 上野川修一
    • Total Pages
      16(446)
    • Publisher
      オーム社(東京)
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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