2005 Fiscal Year Final Research Report Summary
Cardiolipin functions in fish polyunsaturated fatty acid metabolism through plasma lipoproteins
| Project/Area Number |
16580165
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
YAMAGUCHI Toshiyasu Tohoku University, Graduate School of Agricultural Science, Associate Professor, 大学院・農学研究科, 助教授 (90167700)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Cardiolipin / Plasma lipoprotein / Polyunsaturated fatty acids / Docosahexaenoic acid / Cholesterol / HDL / Phospholipids |
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| Research Abstract |
Radioactive docosahexaenoic acid (DHA) was injected using with the fiberscope into the carp intestine and the fatty acid was incorporated into phospholipids. The DHA was immediately detected in the hepatopancreas.
Radioactive docosahexaenoic acid was detected in plasma lipoprotein after injection but radioactive eicosapentaenoic acid (EPA) was detected only small amounts. And DHA was incorporated into leukocyte cardiolipin but EPA and arachidonic acid were not incorporated into carp cardiolipin.
In this research the activities of cholesterol ester transfer protein (CETP) and phospholipid transfer protein (PLTP) were clarified in carp plasma using the radioactive polyunsaturated fatty acid 8PUFA9. Cardiolipin was not detected in fish plasma lipoprotein, but in blood cells especially leukocytes cardiolipin was detected and DHA was incorporated into cardiolipin. It is suggested that cardiolipin was important component for the function of DHA in the fish blood cells.
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