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2006 Fiscal Year Final Research Report Summary

Studies on the colonization mechanism of indigenous bacteria in alimentary tract

Research Project

Project/Area Number 16580239
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKobe University

Principal Investigator

KITAGAWA Hiroshi  Kobe University, Graduate School of Science and Technology, Professor, 自然科学研究科, 教授 (40125307)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Akira  Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (50031224)
Project Period (FY) 2004 – 2006
Keywordsalimentary tract / apoptosis / indigenous bacteria / colonization / Peyer's patch / M cells / sugars / ultrastructure
Research Abstract

This research clarified the colonization mechanisms of indigenous bacteria in rat alimentary tracts. The results obtained in this research are as follows:
1. Fundamental colonization site of indigenous bacteria is the epithelial cells with late apoptotic stage in intestinal villi and Peyer's patches.
2. Differentiation of M cells in the follicle-associated epithelium (FAE) is co-related with the colonization of indigenous bacteria on FAE and neighboring mucosa. The ordinary columnar epithelial cells engulf the degenerated bacteria, whereas M cells engulf the intact bacteria in FAE of Peyer's patches. This engulfment of bacteria might be mediated with membrane receptor in M cells form ultrastructural findings.
3. Specific sugar expression was histochemically determined on the epithelial cells with late apoptotic stage, whereas M cells expressed no specific sugar.
4. Fundamental colonization site of indigenous bacteria is the epithelial cells with late apoptotic stage in entire alimentary tract. From these sites, indigenous bacteria proliferate deeply into mucous membrane. This proliferation is monitored by neighboring FAE of mucous lymphatic follicles.
5. At bacterial attachment sites, indigenous bacteria are eliminated by both the physical defense of rearrangement of epithelial cytoskeletons and the chemical defense of secretion of antibactericidal substances.
6. The detailed differentiation and re differentiation processes were clarified in FAE of Peyer's patches under transmission electron microscope.

  • Research Products

    (16 results)

All 2007 2006 2005 2004

All Journal Article (16 results)

  • [Journal Article] Ultrastructural study on the differentiation and the fate of M cells in follicle-associated epithelium of rat Peyer's patch.2007

    • Author(s)
      Sachiko, Onishi
    • Journal Title

      Journal of Veterinary Medical Science 69・5(in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Persorption mechanisms of luminal antigenic particulates via apoptotic epithelial cells of intestinal villi into systemic blood circulation in orally-immunized rats.2007

    • Author(s)
      Midori, Yuji
    • Journal Title

      Journal of Veterinary Medical Science 69・4(in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Special sugar expression on apoptotic epithelial cells of Peyer's patches and intestinal villi in rat small intestine.2007

    • Author(s)
      Keigi, Chin
    • Journal Title

      Journal of Veterinary Medical Science 69・2

      Pages: 193-199

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ultrastructural study on the differentiation and the fate of M cells in follicle-associated epithelium of rat Peyer's patch.2007

    • Author(s)
      Sachiko, Onishi
    • Journal Title

      Journal of Veterinary Medical Science 69-5 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Persorption mechanisms of luminal antigenic particulates via apoptotic epithelial cells of intestinal villi into systemic blood circulation in orally-immunized rats.2007

    • Author(s)
      Midori, Yuji
    • Journal Title

      Journal of Veterinary Medical Science 69-4 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Special sugar expression on apoptotic epithelial cells of Peyer's patches and intestinal villi in rat small intestine.2007

    • Author(s)
      Keigi, Chin
    • Journal Title

      Journal of Veterinary Medical Science 69-2

      Pages: 193-199

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Persorption of luminal antigenic molecules and its specific antibody via apoptotic epithelial cells of intestinal villi and Peyer's patches into peripheral blood in rats.2006

    • Author(s)
      Midori, Yuji
    • Journal Title

      Journal of Veterinary Medical Science 68・12

      Pages: 1297-1305

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differentiation of epithelial cells to M cells in response to bacterial colonization on the follicle-associated epithelium of Peyer's patch in rat small intestine.2006

    • Author(s)
      Keigi, Chin
    • Journal Title

      Journal of Veterinary Medical Science 68・10

      Pages: 1023-1028

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Persorption of luminal antigenic molecules and its specific antibody via apoptotic epithelial cells of intestinal villi and Peyer's patches into peripheral blood in rats.2006

    • Author(s)
      Midori, Yuji
    • Journal Title

      Journal of Veterinary Medical Science 68-12

      Pages: 1297-1305

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Differentiation of epithelial cells to M cells in response to bacterial colonization on the follicle-associated epithelium of Peyer's patch in rat small intestine.2006

    • Author(s)
      Keigi, Chin
    • Journal Title

      Journal of Veterinary Medical Science 68-10

      Pages: 1023-1028

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of methicillin-resistant Staphylococcus aureus isolated from retail raw chicken meat in Japan.2005

    • Author(s)
      Satoshi, Kitai
    • Journal Title

      Journal of Veterinary Medical Science 67・1

      Pages: 107-110

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Prevalence and characterization of Staphylococcus aureus and enterotoxigenic Staphylococcus aureus in retail raw chicken meat throughout Japan.2005

    • Author(s)
      Satoshi, Kitai
    • Journal Title

      Journal of Veterinary Medical Science 67・3

      Pages: 269-274

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization of methicillin-resistant Staphylococcus aureus isolated from retail raw chicken meat in Japan.2005

    • Author(s)
      Satoshi, Kitai
    • Journal Title

      Journal of Veterinary Medical Science 67-1

      Pages: 107-110

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Prevalence and characterization of Staphylococcus aureus and enterotoxigenic Staphylococcus aureus in retail raw chicken meat throughout Japan.2005

    • Author(s)
      Satoshi, Kitai
    • Journal Title

      Journal of Veterinary Medical Science 67-3

      Pages: 269-274

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Evidence of lactoferrin transportation into blood circulation from intestine via lymphatic pathway in adult rats.2004

    • Author(s)
      Takashi, Takeuchi
    • Journal Title

      Experimental Physiology 89・3

      Pages: 263-270

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Evidence of lactoferrin transportation into blood circulation from intestine via lymphatic pathway in adult rats.2004

    • Author(s)
      Takashi, Takeuchi
    • Journal Title

      Experimental Physiology 89-3

      Pages: 263-270

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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