2005 Fiscal Year Final Research Report Summary
Super-high Efficient Foreign Protein Expression System in Plants
| Project/Area Number |
16580282
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | ISHIKAWA PREFECTURAL UNIVERSITY |
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Principal Investigator |
MORI Masashi Ishikawa Prefectural University, Faculty of Bioresources and Environmental Sciences, Associate Professor, 生物資源環境学部, 助教授 (00320911)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Plant Virus Vector / Silencing Suppressor / Transgenic Plants / Foreign Gene Expression |
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| Research Abstract |
The aim of this study was to establish super-high efficient foreign protein expression system in plants by controlled expression of a plant virus based vector and silencing suppressors, which prevents specific degradation of viral vector RNA in a later stage of the infection. Genes of three silencing suppressors, cucumber mosaic virus 2b, tomato bushy stunt virus p19 and tomato spotted wilt virus. NSs, which suppressed gene silencing by possibly different mechanisms, were introduced to estradiol-and dexamethasone-inducible expression vectors, and transformed into Nicotiana benthamiana plants. Transgenic plants that inducibly expressed one of these suppressors were successfully produced. However, the expression of the suppressors in the cross-progenies that had two or three different suppressors were often significantly suppressed, suggesting a synergistic adverse effect of these suppressors. Next, transgenic plants that inducibly expressed one of the silencing suppressors and a replicable brome mosaic virus (BMV)-based vector by treatment of dexamethasone were successfully produced by crossing of the transgenic plants. Significantly, in the transgenic plants that inducibly expressed NSs and the BMV vector, the decreasing of the viral RNA and the foreign protein encoded in the vector, which was usually observed in a later stage of the infection, was suppressed. Various useful proteins may be produced in transgenic plants that inducibly express NSs and a BMV vector.
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Research Products
(14 results)
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[Journal Article] Membrane-bound tomato mosaic virus replication proteins participate in RNA synthesis and are associated with host proteins in a pattern distinct from that observed for non-membrane-bound ones2006
Author(s)
Nishikiori, M., Dohi, K., Mori, M., Meshi, T., Naito S., Ishikawa,M.
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Journal Title
Journal of Virology 80
Pages: 8459-8468
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Expression of an enhancin gene from Trichoplusia ni granulosis virus confers the resistance to lepidopterous insect pests to rice2006
Author(s)
Mori, M., Kitamura, H., Kondo, A., Dohi, K., Mori, M., Kaido, M., Mise, K., Shimojyo, E., Hashimoto,Y.
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Journal Title
Plant Biotechnology 23
Pages: 55-61
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Inducible virus-mediated expression of a foreign protein in suspension-cultured plant cells2006
Author(s)
Dohi, K., Nishikiori, M., Tamai, A., Ishikawa, M., Meshi, T., Mori,M
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Journal Title
Archives of Virology 151
Pages: 1075-1084
Description
「研究成果報告書概要(和文)」より
-
[Journal Article] Membrane-bound tomato mosaic virus replication proteins participate in RNA synthesis and are associated with host proteins in a pattern distinct from that observed for non-membrane-bound ones.2006
Author(s)
Nishikiori, M., Dohi, K., Mori, M., Meshi, T., Naito S., Ishikawa, M.
-
Journal Title
Journal of Virology 80
Pages: 8459-8468
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Expression of an enhancin gene from Trichoplusia ni granulosis virus confers the resistance to lepidopterous insect pests to rice.2006
Author(s)
Mori, M.Kitamura, H., Kondo, A., Dohi, K., Mori, M., Kaido, M., Mise, K., Shimojyo, E., Hashimoto, Y.
-
Journal Title
Plant Biotechnology 23
Pages: 55-61
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Inducible virus-mediated expression of a foreign protein in suspension-cultured plant cells.2006
Author(s)
Dohi, K., Nishikiori, M., Tamai, A., Ishikawa, M., Meshi, T., Mori, M.
-
Journal Title
Archives of Virology 151
Pages: 1075-1084
Description
「研究成果報告書概要(欧文)」より
-
-
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[Journal Article] Downregulation of the NbNACal gene encoding a movement-protein-interacting protein reduces cell-to-cell movement of Brome mosaic virus in Nicotiana benthamiana.
Author(s)
Kaido, M., Inoue, Y., Takeda, Y., Sugiyama, K., Takeda, A., Mori, M., Tamai, A., Meshi, T., Okuno, T., Mise,K.
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Journal Title
Molecular Plant-Microbe Interactions (In press)
Description
「研究成果報告書概要(和文)」より
-
[Journal Article] Downregulation of the NbNACal gene encoding a movement-protein-interacting protein reduces cell-to-cell movement of Brome mosaic virus in Nicotiana benthamiana.
Author(s)
Kaido, M., Inoue, Y., Takeda, Y., Sugiyama, K., Takeda, A., Mori, M., Tamai, A., Meshi, T., Okuno, T., Mise, K.
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Journal Title
Molecular Plant-Microbe Interactions (in press)
Description
「研究成果報告書概要(欧文)」より
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