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2005 Fiscal Year Final Research Report Summary

Development of simultaneous measurement of micro/macro-molecular kinetics in a living cell by laser speckle microscopy

Research Project

Project/Area Number 16590028
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

HIRAKAWA Yasuyuki  Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (80238344)

Co-Investigator(Kenkyū-buntansha) MASUJIMA Tutomu  Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (10136054)
Project Period (FY) 2004 – 2005
KeywordsCell Observation / Laser Speckle / Cellular Activity / Fluorescence Observation
Research Abstract

A laser speckle microscopy imaging system for observation of living cells was developed. This system consists of an optical microscope, a laser source, and a video camera. The system has an advantage in that cellular conditions could be instantaneously determined by observing speckle movements without any special preparation such as staining and genetic manipulation. In the experiments, the speckle fluctuations of living and fixed HeLa cells were compared with the change in the temperature of the culture medium around the cells, and their video images were quantitatively analyzed. Laser speckle fluctuations on these cells were also analyzed by using frequency spectra of speckle fluctuations. In this frequency analysis, particular peak around a few Hz was observed in a living cell. It was also found in this study that by using a femto second laser illumination, simultaneous observation of auto-fluorescence from bio-molecules and laser speckle in a living cell could be possible in this laser speckle microscopic system. By applying this system, it is possible to estimate not only cellular activity but also real-time movements of intercellular materials including the cytoplasm without requiring complicated labeling processes.

  • Research Products

    (5 results)

All 2006 2005

All Journal Article (4 results) Book (1 results)

  • [Journal Article] Recent Progress of Laser Microscopes2006

    • Author(s)
      Y.Hirakawa
    • Journal Title

      Recent Developments in Lasers and their Applications (N.Vasa eds.)(Research Signpost)

      Pages: 135-166

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Laser Speckle Microscope for Instanteneous Determination of Condition of Single Living Cell2005

    • Author(s)
      Y.Hirakawa
    • Journal Title

      Japanese Journal of Applied Physics 44・2

      Pages: L85

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Frequency Analysis of Single Living Cell Activity by Laser Speckle Microscope2005

    • Author(s)
      Y.Hirakawa
    • Journal Title

      Technical Digest of Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications, Systems and Technologies 2005

      Pages: CThC6

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Laser Speckle Microscope for Instanteneous Determination of Condition of Single Living Cell2005

    • Author(s)
      Y.Hirakawa
    • Journal Title

      Japanese Journal of Applied Physics 44(2)

      Pages: L85

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Recent Progress of Laser Microscopes in Recent Developments in Lasers and their Applicaiotions (Chapter 7)2006

    • Author(s)
      Yasuyuki Hirakawa (N. Vasa eds.)
    • Total Pages
      32pages(Ch.7)
    • Publisher
      Research Signpost
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2007-12-13  

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