2004 Fiscal Year Final Research Report Summary
Fundamental researches for antibody medicine development by the virus vector aiming at control of the immune response
| Project/Area Number |
16590086
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
ITO Yuji Kagoshima University, Faculty of Engineering, Associate Professor (60223195)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIMUEA Kazuhisa Kagoshima University, Faculty of Engineering, Professor (80127240)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Antibody phage library / immune response control / Sendai virus vector / costimulatory molecule / gene expression / antibody medicine / gene therapy |
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| Research Abstract |
The autoimmune disease such as allergy or the rheumatoid arthritis is caused by failure of normal maintenance m chanism of the immune system. The control of the T cell activation as well as anti-inflammation medical treatment is effective for the treatment of such patients. Furthermore,in even the prevention and treatment of cancer or AIDS by vaccine,the control of the immunoresponse is a very important issues owing to raising the effectiveness of the vaccine.
The purpose of this study is to evaluate the control of the T cell response using the human antibody specific to costimulatory molecules which is carried by Sendai virus vector,which would give an important strategy for the effective vaccine development of autoimmune disease treatment,cancer and AIDS vaccine.
In this study,we aimed at establishing the system which controlled the activation of T cell,B cell,the antigen presenting cell by targeting of the antibody to an immune cells and controlling the costimulatory signal.We examined the change of the costimulatory signal by the addition of the antibody isolated from antibody phage library and also the influence on the immune response by T cell proliferation with human peripheral monocyte (PBMC). Subsequently,we constructed the Sendai virus vector which carried an antibody gene and tried to analyze the immune response by antibody which was produced by the Sendai virus-infected cells. However, as a result,we could not achieve the original purpose,because the very low production of the objective antibody was seen from an animal cell after the Sendai viral infection. As a reason for the low production of antibody,the low stability of the human antibody used here was suggested. Therefore,we decided to precede the study,improving the stabilization of the antibody by protein engineering.
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