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2006 Fiscal Year Final Research Report Summary

Effect of Kosa on Th1 and Th2 immune responses and on oral tolerance

Research Project

Project/Area Number 16590099
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionKobe Pharmaceutical University

Principal Investigator

YOSHINO Shin  Kobe Pharmaceutical University, Pharmacy, professor, 薬学部, 教授 (00260729)

Project Period (FY) 2004 – 2006
KeywordsKosa / Th1 / Th2 / oral tolerance / cytokine / allergy
Research Abstract

The effects of Kosa on Th1 and Th2 immune responses and on oral tolerance in mice were investigated. Th1 responses appear to be involved in some of autoimmune diseases such as rheumatoid arthritis and Th2 in allergic diseases including pollen-induced rhinitis. Oral tolerance appears to play an important role in preventing food allergy. To induce Th1 and Th2 immune responses, DBA mice were immunized with ovalbumin (OVA) (day 0). On day 21, anti-OVA IgG2a antibodies and IFN-g were measured as indicators of Th1 immune responses and anti-OVA IgG1 and IgE antibodies and IL-4 as those of Th2 immune responses. TGF-b and IL-10 were also measured as indicators Th3-and regulatory T cell-mediated immune responses, respectively. Th1 and Th2 oral tolerance were induced by feeding mice 20 and 0.1 mg of OVA, respectively, from days-5 to-1. Varying doses of Kosa were orally administered daily over a period of 21 days commencing on day 0 to test its effects on Th1 and Th2 responses. To examine the effect of Kosa on oral tolerance, Kosa were orally given daily from days-5 to -1. PBS was fed as a control. The results showed that Kosa failed to affect production of anti-OVA IgG2a and IFN-g. In contrast, anti-OVA IgE and IL-4 secretion was facilitated by Kosa, although anti-OVA IgGl production was unaffected by Kosa. There was no difference in production of TGF-b and IL-10 between Kosa and PBS-treated mice. Th2 but not Th1 oral tolerance appeared to be enhanced by Kosa. These results suggest that Kosa may facilitate Th2 but not Th1 immune responses. Th2 but not Th1 oral tolerance also may be enhanced by Kosa. The function of Th3 and regulatory T cells does not appear to be affected by Kosa.

  • Research Products

    (10 results)

All 2006 2005 2004

All Journal Article (10 results)

  • [Journal Article] Suppression of Th1 and Th2 immune responses in mice by sinomenine2006

    • Author(s)
      Feng Huang
    • Journal Title

      Planta Medica 72

      Pages: 1383-1388

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Suppression of Th1 and Th2 immune responses in mcie by sinomene2006

    • Author(s)
      Feng Huang
    • Journal Title

      Planta Medica 72

      Pages: 1383-1388

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of varying types of anti-arthritic drugs on Th1 and Th2 immune responses in mice2005

    • Author(s)
      Kouya Yamaki
    • Journal Title

      International Journal of Immunopharmacology and Pharmacology 18

      Pages: 133-144

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reactivation of antigen-induced arthritis in mice by Oral administration of lipopolysaccharide2005

    • Author(s)
      Shin Yoshino
    • Journal Title

      Scandinavian Journal of Immunology 62

      Pages: 117-122

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of rolipram, a phosphodiesterase IV inhibitor, on allergic footpad swelling using varying adjuvants2005

    • Author(s)
      Kouya Yamaki
    • Journal Title

      Scandinavian Journal of Immunology 62

      Pages: 378-384

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reactivation of antigen-induced arthritis in mice by oral administration of lipopolysaccharide2005

    • Author(s)
      Shin Yoshino
    • Journal Title

      Scandinavian Journal of Immunology 62

      Pages: 117-122

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel water-soluble vitamin E derivative prevents acute lung injury by bacterial endotoxin2004

    • Author(s)
      Kazuhiko Uchiyama
    • Journal Title

      Clinical and Experimental pharmacology and Physiology 31

      Pages: 226-230

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of the phosphodiesterase IV inhibitor rolipram On Th1 and Th2 immune responses in mice2004

    • Author(s)
      Kouya Yamaki
    • Journal Title

      Journal of Pharmacy and Pharmacology 56

      Pages: 877-882

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A novel water-soluble vitamine E derivative prevents acute lung injury by bacterial endotoxin2004

    • Author(s)
      Kazuhiko Uchiyama
    • Journal Title

      Clinical and Experimental Pharmacology 31

      Pages: 226-230

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of the phosphodiesterase IV inhibitor rolipram on Th1 and Th2 immune responses in mice2004

    • Author(s)
      Kouya Yamaki
    • Journal Title

      Journal of Pharmacy and Pharmacology 56

      Pages: 877-882

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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