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2005 Fiscal Year Final Research Report Summary

MafA differentiates rat intestinal cells into insulin-producing cells.

Research Project

Project/Area Number 16590143
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionShiga University of Medical Science

Principal Investigator

NAKAMURA Takaaki  Shiga University of Medical Science, Medicine, Assistant Professor, 医学部, 助手 (30314157)

Co-Investigator(Kenkyū-buntansha) KASHIWAGI Atsunori  Shiga University of Medical Science, Medicine, Professor, 医学部, 教授 (20127210)
KUDO Motoi  Shiga University of Medical Science, Medicine, Professor, 医学部, 教授 (80108141)
Project Period (FY) 2004 – 2005
Keywordstranscription factor / Mafa / rat intestine / diabetes mellitus / Pdx1 / insulin / gene therapy
Research Abstract

Recent studies have suggested that basic leucine zipper transcription factor MafA has a crucial role in pancreatic □-cell-specific insulin gene transcription. Thus, we investigated whether MafA overexpression in the intestine induces insulin production in small-intestinal epithelial cells in vivo. Recombinant adenovirus containing MafA gene(Ad-MafA) was prepared and administered orally to streptozocin-treated diabetic rats. Insulin gene expression was observed in the intestine by RT-PCR analysis, and then insulin protein was detected by immunohistochemical analysis after Ad-MafA administration. Furthermore, MafA overexpression in the intestine increased plasma insulin levels and ameliorated hyperglycemia. These results indicate that MafA overexpression in the intestine induces intestinal epithelial cells newly to produce and release insulin.

  • Research Products

    (4 results)

All 2005 2003 Other

All Journal Article (4 results)

  • [Journal Article] Regulation of ATP-sensitive potassium channel subunit Kir6.2 Expression in rat intestinal insulin producing progenitor cells.2005

    • Author(s)
      Tetsuya Hashimoto, Takaaki Nakamura, et al.
    • Journal Title

      The Journal of Biological Chemistry 280

      Pages: 1893-1900

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Diet high in lipid hydroperoxide by vitamin E deficiency induces insulin resistance and impaired insulin secretion in normal rats.2005

    • Author(s)
      K Tsujinaka, Takaaki Nakamura
    • Journal Title

      Diabetes Research and Clinical i 67

      Pages: 99-109

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sumoylation of Pdx1 is associated with its nuclear localization and insulin gene activation.2003

    • Author(s)
      Akio Kishi, Takaaki Nakamura, et al.
    • Journal Title

      Am J Physiol Endocrinol Metab 284

      Pages: E830-E840

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] MafA differentiates rat intestinal cells into insulin-producing cells

    • Author(s)
      Satoshi Nomura, Takaaki Nakamura, et al.
    • Journal Title

      Biochemical and Biophysical Research Communications (投稿中)

    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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