2005 Fiscal Year Final Research Report Summary
Dynamic analysis of tight junction strands
| Project/Area Number |
16590155
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | The Jikei University School of Medicine |
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Principal Investigator |
SASAKI Hiroyuki The Jikei University School of Medicine, Assistant Professor, 医学部, 助教授 (60170693)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Tight junction / Claudin / GFP / Tet-Off |
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| Research Abstract |
The tight junction (TJ) strand is a linear polymer, typical of proteinaceous polymers within plasma membranes, and the TJ strand itself is thought to be involved directly in the barrier and fence functions of TJs. I constructed the plasmid for expression of a fusion protein of the full length of claudin-1,the green fluorescent protein (GFP) and cytoplasmic COOH domain of claudin-1,and transfected the plasmid into Tet-Off cells derived from L type fibroblast cells and Madin-Darby Canine Kidney I cells. Then, I attempted to visualize individual TJ strands in the fibroblast or epithelial cells. These studies would reveal to what extent the behavior of the in situ TJ strands is dynamic as compared to the reconstituted paired claudin strands, and how the dynamic characteristics of the TJ strands are modified and/or regulated in epithelial/endothelial cells.
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