2005 Fiscal Year Final Research Report Summary
Mechanism of the cytoskeletal regulation of store-operated Ca^<2+> entry
| Project/Area Number |
16590165
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
OMATSU Mariko Shiga University of Medical Science, Assistant Professor, 医学部, 助教授 (80161397)
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| Project Period (FY) |
2004 – 2005
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| Keywords | store-operated Ca^<2+> entry / P2 receptor / ATP / intracellular Ca^<2+> / cytoskeleton / actin / endoplasmic reticulum / brown adipocyte |
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| Research Abstract |
Stimulation of P2 receptors with micromolar concentration of ATP not only evokes a transient increase in intracellular free Ca^<2+> concentration ([Ca^<2+>]_i), primarily due to release of Ca^<2+> from intracellular stores ; such stimulation also triggers almost complete suppression of thapsigargin-evoked sustained [Ca^<2+>]_i elevation mediated through a store-operated Ca^<2+> entry pathway in rat brown adipocytes. We investigated the role of cytoskeletal actin in the inhibitory effect which extracellular ATP has on store-operated Ca^<2+> entry, using fura-2 fluorescence for continuous measurement of [Ca^<2+>]_i_, and using Alexa fluor 488-phalloidin staining of actin. Disassembly of actin networks by cytochalasin D (1 μM) or latrunculin A (3 μM) prevented the inhibitory effect of ATP (10 μM) on the thapsigargin (100 μM)-evoked store-operated Ca^<2+>entry, without changing the effect of ATP in raising [Ca^<2+>]_i. In normal cells, bath application of ATP induced a transient [Ca^<2+>]_i elevation consisting of a rapid increase (the rising phase) and the subsequent decrease (the declining phase) to a lower steady level despite the continued presence of the agonist. Disruption of actin assemblies did not significantly affect the rising phase, but prevented the declining phase. Cells incubated with 10 μM ATP for 4 min demonstrated marked accumulations of actin filaments at the cell periphery, showing protrusions at the cell surface ; this actin-assembly process is mediated through P2 receptors. In cells treated with cytochalasin D or latrunculin A, extracellular ATP did not induce actin redistribution. These results suggest the actin reorganization plays a role in ATP-induced inhibition of store-operated Ca^<2+> entry in rat brown adipocytes.
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Research Products
(16 results)
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[Journal Article] Invitro expression of natriuriuretic peptides in cardiomyocytes differentiated from monkey embryonic stem cells.2006
Author(s)
Abdelalim, E.M, Takada, T., Toyoda, F., Omatsu-Kanbe, M., Matsuura, H., Tooyama, I., Torii,R
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Journal Title
Biochem.Biophys.Res.Commun 340
Pages: 689-695
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Angiotensin II potentiates the slow component of delayed rectifier K^+ current via the AT 1 receptor in guinea pig atrial myocytes.2006
Author(s)
Zankov, D.P., Omatsu-Kanbe, M, Isono, T., Toyoda, F., Ding, W.g., Matsuura, H, Horie,M.
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Journal Title
Circulation 113
Pages: 1278-1286
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Effect of PKCβ inhibitor on Ca^<2+> homeostasis I diabetic sensory neurons2006
Author(s)
Tahara, M., Omatsu-Kanbe, M., Sanada, M., Maeda, K., Koya, D., matsuura, H., Kashiwagi, A., Yasuda,H.
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Journal Title
Neuroreport 17
Pages: 683-688
Description
「研究成果報告書概要(和文)」より
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-
[Journal Article] In vitro expression of natriuretic peptides in cardiomyocytes differentiated from monkey embryonic stem cells.2006
Author(s)
Abdelalim, E.M., Takada, T., Toyoda, F., Omatsu-Kanbe, M., Matsuura, H., Tooyama, I., Torii, R.
-
Journal Title
Biochem.Biophys.Res.Commun. 340
Pages: 689-695
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Angiotensin II potentiates the slow component of delayed rectifier K^+ current via the AT 1 receptor in guinea pig atrial myocytes.2006
Author(s)
Zankov, D.P., Omatsu-Kanbe, M., Isono, T., Toyoda, F., Ding, W.G., Matsuura, H., Horie, M.
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Journal Title
Circulation 113
Pages: 1278-1286
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of PKCb inhibitor on Ca^<2+> homeostasis I diabetic sensory neurons2006
Author(s)
Tahara, M., Omatsu-Kanbe, M., Sanada, M., Maeda, K., Koya, D., Matsushita, H., Kashiwagi, A., Yasuda, H.
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Journal Title
Neuroreport 17
Pages: 683-688
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cytosolic Ca^<2+> under high glucose with suppressed Na+/K+ pump activity in rat sensory neurons.2004
Author(s)
Sanada, M., Matsuura, H., Omatsu-Kanbe, M., Sango, K., Kashiwagi, A., Yasuda,H.
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Journal Title
Neuroreport 15
Pages: 197-201
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Identification of endogenous surrogate legend for human P2Y receptors through an insilico search.2004
Author(s)
Hiramoto, T., Nonaka, Y., Yamamoto, T., Omatsu-Kanbe, M., Matsuura, H., Gohda, K., Fujita, N.
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Journal Title
J.Pharmacol.Sci. 95
Pages: 81-93
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cytosolic Ca^<2+> under high glucose with suppressed Na^+/K^+ pump activity in rat sensory neurons.2004
Author(s)
Sanada, M., Matsuura, H., Omatsu-Kanbe, M., Sango, K., Kashiwagi, A., Yasuda, H.
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Journal Title
Neuro Report 15
Pages: 197-201
Description
「研究成果報告書概要(欧文)」より
