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2005 Fiscal Year Final Research Report Summary

Effect of environmental stress responsive transcription factor ATF5 on adipogenesis

Research Project

Project/Area Number 16590179
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionTokyo University of Pharmacy & Life Sciences

Principal Investigator

TAKAHASHI Shigeru  Tokyo University of Pharmacy & Life Sciences, School of Life Science, Associate Professor, 生命科学部, 助教授 (10266900)

Project Period (FY) 2004 – 2005
KeywordsATF5 / adipocyte / differenciation / adeno virus vector / gene expression / transcription factor
Research Abstract

Obesity is a risk factor of metabolic syndrome including diabetes, hypertension and hyperlipidemia. Obesity is caused by an excessive adipocytes and related to the cell growth and differentiation of adipocytes. On the other hand, I have found that ATF5 mRNA levels decreases to 30% according to the adipocyte differentiation. This suggests that ATF5 might have a significant function on the adipocyte differentiation. I have found that ATF5 mRNA expression is up-regulated by a variety of stresses. This shows that changes of ATF5 expression by the stresses might disturb the expression patterns of the target genes for ATF5 and leads to the pathological status. Until now, the genes whose expression are regulated by ATF5 are unknown. The exploration of those genes is one of the useful ways for elucidating the functions of ATF5. I planed to knock down the expression of ATF5 in adipocyte using SiRNA technique, and compare the expression pattern of a variety of genes between in knockdown cells and in control cells with DNA microarray analysis. At first, I tried to produce the effective SiRNA for ATF5 expression. I checked the effeciency of the SiRNA using the cells which transfected with the flag-ATF5 exprerssion plasmid, the expression of flag-ATF5 protein was effectively repressed. Furthermore, to express ATF5 in cells exogenously, I tried to construct the adeno virus vector.

  • Research Products

    (4 results)

All 2005 Other

All Journal Article (4 results)

  • [Journal Article] Transcriptional co-activator CSA regulates chondrogenesis via association with SOX92005

    • Author(s)
      Kawakami, Y, et al.
    • Journal Title

      PROC.Natl.Acad.Sci.USA 102

      Pages: 2414-2419

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transcriptional co-activator CSA regulates chondrogenesis via association with SOX92005

    • Author(s)
      Kawakami, Y. et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 102

      Pages: 2414-2419

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Tissue preferential expression of estrogen receptor gene in marine snail,Thais clavigera

    • Author(s)
      Kajiwara, M, et al.
    • Journal Title

      Gen.Com.Endocrinol. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tissue preferential expression of estrogen receptor gene in the marine snail, Thais clavigera

    • Author(s)
      Kajiwara, M. et al.
    • Journal Title

      Gen.Com.Endocrinol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

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