• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

Functions of the Maf transcription factors in development and differentiation

Research Project

Project/Area Number 16590215
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

SAKAI Masaharu  Hokkaido University, School of Medicine, Professor, 医学部, 教授 (50162269)

Co-Investigator(Kenkyū-buntansha) IMAKI Junko  National Defense Medical College, Professor, 教授 (20223323)
Project Period (FY) 2004 – 2005
KeywordsChondrocyte / Cellular differentiation / Transcription factors / Maf / CTGF / GST-P / C / EBP / DNA micro-array
Research Abstract

1,c-Maf regulates CTGF gene ; We have previously reported that the c-Maf and MafB transcription factors were specifically expressed in the differentiation process of lens, cartilage, spinal cord and kidney. However the target genes and functions in these tissues are not known. Introduction of the Maf gene into the fibroblast cell line using adenovirus vector induced connective tissue growth factor (CTGF) gene, which has pivotal functions in cartilage development. From the detailed analyses, including EMSA, reporter transfection and ChIP analyses, c-Maf binds to the promoter region and 5'-untranslated regions of CTGF gene and strongly activates this gene. (Biochem Biophys Res Commn 339,1089-1097,2006)
2.Identification of c-Maf target genes by DNA micro-array analysis : To identify the target genes of c-Maf, we have performed DNA micro-array analysis using the mouse embryonic fibroblast (MEF) cells from wild and c-maf knock-out mice. We identified several genes, including fibroblast growth factors (Fgf 18 and Fgf 9), which significantly reduced the expression in KO-MEF cells. The detailed analyses on these genes are currently in progress.
3,Regulation of glutathione S-transferase (GST-P) gene during hepatocarcinogenesis. GST-P is a tumor marker activated by the Nrf2/MafK hetero-dimers in early stage of carcinogenesis (Biochemical J.380,515-521,2004). Recently, we identified the CAAT enhancer binding protein α (C/EBPα) as a suppressor of this gene in the normal liver. C/EBPα specifically binds to the GPE1,a strong enhancer element of GST-P gene, in vitro and in vivo. C/EBPα expressed in normal liver and strongly inhibited the Nrf2/MafK activity by competing the binding site (GPE1) with the Nrf2/MafK. In the early stage of carcinogenesis, the C/EBPα expression is completely shut-off, and the substitution of GPE1 binding factor from C/EBPα to the Nrf2/MafK lead to the strong induction of GST-P expression. (J.Biol.Chem.in press)

  • Research Products

    (19 results)

All 2006 2005 2004 Other

All Journal Article (19 results)

  • [Journal Article] Activation of Connective Tissue Growth Factor Gene by the c-Maf and Lc-Maf Transcription Factors.2006

    • Author(s)
      Omoteyama K. et al.
    • Journal Title

      Biochem Biophys Res Commun. 339

      Pages: 1089-1097

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] CCAAT enhancer binding protein α(C/EBP α) suppresses the rat placental glutathione S-transferase gene in normal liver.2006

    • Author(s)
      Ikeda, H. et al.
    • Journal Title

      J. Biol. Chem. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunolocalization of cyclin D1 in the developing lens of c-maf -/- mice.2006

    • Author(s)
      Kase S.et al.
    • Journal Title

      Acta Histochem 107

      Pages: 469-472

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Interleukin-6 and Mevastatin Regulate Plaminogen Activator Inhibitor-1 Through CCAAT/Enhancer-Binding Protein-δ.2005

    • Author(s)
      Dong, J. et al.
    • Journal Title

      Arterioscler Thrormb Vasc Biology 25

      Pages: 1078-1084

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunolocalization of cyclin D1 in the developing lens of c-maf -/- mice.2005

    • Author(s)
      Kase S.et al.
    • Journal Title

      Acta Histochem. 107

      Pages: 469-472

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of GST-P gene expression during hepatocarcinogenesis.2005

    • Author(s)
      Sakai.M.et al.
    • Journal Title

      Methods in Enzymology (Elsevier) vol. 401

      Pages: 20

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of GST-P gene expression during hepatocarcinogenesis.2005

    • Author(s)
      Sakai M., et al.
    • Journal Title

      Methods in Enz. 401

      Pages: 42-61

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Interleukin-6 and Mevastatin Regulate Plaminogen Activator Inhibitor-1 Through CCAAT/Enhancer-Binding Protein-δ.2005

    • Author(s)
      Dong J.et al.
    • Journal Title

      Arterioscler Thrormb Vasc Biol. 25

      Pages: 1078-1084

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of c-maf and mafB genes in the skin during rat embryonic development.2004

    • Author(s)
      Ogata A. et al.
    • Journal Title

      Acta histochemica 106

      Pages: 65-67

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transcription factor Nrf2/MafK regulates rat placental glutathione S-transferase gene during hepatocarcinogenesis.2004

    • Author(s)
      Ikeda, H. et al.
    • Journal Title

      Biochemical J 380

      Pages: 515-521

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Functional mapping of tissue-specific elements of the human alpha-fetoprotein gene enhancer.2004

    • Author(s)
      Nakabayashi, H. et al.
    • Journal Title

      Biochem Biophys Res Commun 318

      Pages: 773-785

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Developmental contribution of c-maf in the kidney : distribution and developmental study of c-maf mRNA in normal mice kidney and histological study of c-maf knock out mice kidney and liver.2004

    • Author(s)
      Imaki J. et al.
    • Journal Title

      Biochem Biophys Res Commun., 320

      Pages: 1323-1327

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of maf-B mRNA in the epithelium around the eyelid closure of the mouse eye at embryonic day 18.2004

    • Author(s)
      Kase S.et al.
    • Journal Title

      Anat Embryol 209・2

      Pages: 153-156

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of c-maf and mafB genes in the skin during rat embryonic development.2004

    • Author(s)
      Ogata, A. et al.
    • Journal Title

      Acta histochemica. 106

      Pages: 65-67

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transcription factor Nrf2/MafK regulates rat placental glutathione S-transferase gene during hepatocarcinogenesis.2004

    • Author(s)
      Ikeda, H. et al.
    • Journal Title

      Biochemical J. 380

      Pages: 515-521

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional mapping of tissue-specific elements of the human alpha-fetoprotein gene enhancer.2004

    • Author(s)
      Nakabayashi H, et al.
    • Journal Title

      Biochem Biophys Res Commun. 318

      Pages: 773-785

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Developmental contribution of c-maf in the kidney : distribution and developmental study of c-maf mRNA in normal mice kidney and histological study of c-maf knockout mice kidney and liver.2004

    • Author(s)
      Imaki J, et al.
    • Journal Title

      Biochem Biophys Res Commun 320

      Pages: 1323-1327

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of maf-B mRNA in the epithelium around the eyelid closure of the mouse eye at embryonic day 18.2004

    • Author(s)
      Kase S, et al.
    • Journal Title

      Anat Embryol (Berl). 209

      Pages: 153-156

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] CCAAT enhancer binding protein α (C/EBP α) suppresses the rat placental glutathione S-transferase gene in normal liver.

    • Author(s)
      Ikeda H. et al.
    • Journal Title

      J.Biol.Chem. (in press)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi