2005 Fiscal Year Final Research Report Summary
Molecular structure, evolution and mechanism of genomic imprinting
| Project/Area Number |
16590232
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Saga University |
|---|
Principal Investigator |
MUKAI Tsunehiro Saga University, Director, 理事 (40108741)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Keywords | Imprinting / Imprinting center / DNA methylation / Murr1 / U2af1-rs1 |
|---|
| Research Abstract |
Human MURR1 is an orthologue of mouse Murr1 gene, which was previously reported to be imprinted only in adult brain with a maternal allele-predominant expression and to contain another imprinted gene, U2af12-rs1, in the first intron. Human MURR1 was found not to harbor the U2af1-rs1 orthologue and to be expressed biallelically in tissues, including adult brain. Three genes, identified around Murr1 and their orthologues around MURR1 were expressed biallelically. These findings suggest that the mouse imprinting locus is limited to a small region and the introduction of U2af1-rs1 in mouse causes the imprinting of this locus. The CpG island(CGI) at U2af1-rs1 with maternal methylation was the only differentially methylated region among CGIs found in these loci. Detailed methylation analyses of the U2af1-rs1 CGI in germ cells led to identification of a region with oocyte-specific methylation. These results suggest that this region is the imprinting control region of the Murr1/U2af1-rs1 locus in mouse. The methylation status of mouse U2af1-rs1 was examined during early stage of embryo and showed to be methylated maternally. The status of histone modification was examined using primary fibroblast cell. The Chip assay was performed using various histone antibodies (against H3Ac, H4Ac, H3mK4) and showed paternally to be active.
|
Research Products
(6 results)
