2005 Fiscal Year Final Research Report Summary
Analysis of correlation between VCP expression and metastasis in human cancers
| Project/Area Number |
16590276
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Osaka University |
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Principal Investigator |
TOMITA Yasuhiko Osaka Univ., Grad.Sch.Med., Assoc.Prof., 医学系研究科, 助教授 (60263266)
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| Co-Investigator(Kenkyū-buntansha) |
HOSHIDA Yoshihiko Osaka Univ., Hosp., Assoc.Prof., 医学部附属病院, 助教授 (40324777)
AOZASA Katsuyuki Osaka Univ., Grad.Sch.Med., Prof., 医学系研究科, 教授 (30115985)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Valosin-containing protein / metastasis / transcription factor / prognosis |
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| Research Abstract |
The researchers have identified that Valosin-containing protein (VCP) to be correlated with the metastasis of a murine osteosarcoma cell line by using the mRNA subtraction technique. This project was designed to identify whether or not VCP expression level in cancer cells was correlated with the rate of recurrence and prognosis of patients with cancer and clarify the transcriptional mechanism of VCP. The result showed that VCP expression is correlated with the progression and prognosis of cancers arising from lung, prostate, pancreatic endocrine, esophagus, gingiva, and thyroid. (Ann.Surg.Oncol.2004;11:697-704,Clin.Cancer Res.2004;10:3007-3012,Oncol.2004;66:468-475, Ann. Oncol. 2004;15:1432-1438,Clin.Cancer. Res.2004;10:5558-5565, Ann. Surg. Oncol. 2005;12:925-934) Transcriptional activity of VCP gene was analyzed by Luciferase reporter assay. Luciferase reporter assay revealed two candidate transcription factors, PBX1 and ELF2 which might be important in VCP transcription. Chromatin immunoprecipitation assay revealed the binding of PBX1 and ELF2 to the 5' flanking region DNA sequence. Cellular knock-down of PBX1 and ELF2 expression with siRNA transfection led decrease and increase of VCP expression, respectively. These findings indicate that PBX1 and ELF2 are important transcription factors for VCP expression.
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[Journal Article] Prognostic significance of BACH2 expression in diffuse large B-cell lymphoma.2005
Author(s)
Sakane-Ishikawa E, Nakatsuka S, Tomita Y, Fujita S, Nakamichi I, Takakuwa T, Sugiyama H, Fukuhara S, Hino M, Kanamaru A, Soma T, Tsukaguchi M, Igarashi K, Kanakura Y, Aozasa K, Osaka Lymphoma Study Group
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Journal Title
J Clin Oncol 23
Pages: 8012-8017
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Increased expression of valosin containing protein (VCP : p97), a member of the ATPase, is associated with lymph node metastasis in pancreatic adenocarcinoma.2004
Author(s)
Yamamoto S, Tomita Y, Hoshida Y, Nagano H, Dono K, Umeshita K, Sakon M, Ishikawa O, Ohigashi H, Nakamori S, Monden M, Aozasa K.
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Journal Title
Ann.Surg.Oncol. 11
Pages: 697-704
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression level of Valosin-containing protein (VCP) is associated with progression and prognosis of esophageal carcinoma.2004
Author(s)
Yamamoto S, Tomita Y, Hoshida Y, Iizuka N, Kidogami S, Takiguchi Y, Fujiwara Y, Yasuda T, Yano M, Nakamori S, Sakon M, Monden M, Aozasa K.
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Journal Title
Clin.Cancer Res. 10
Pages: 5558-5565
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression level of Valosin-containing protein (VCP) as a prognostic marker for Gingival Squamous Cell Carcinoma.2004
Author(s)
Yamamoto S, Tomita Y, Hoshida Y, Toyosawa S, Inohara H, Kishino M, Kogo M, Nakazawa M, Murakami S, Iizuka N, Kidogami S, Monden M, Kubo T, Ijuhin N, Aozasa K.
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Journal Title
Ann.Oncol. 15
Pages: 1432-1438
Description
「研究成果報告書概要(欧文)」より
