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2005 Fiscal Year Final Research Report Summary

How malaria parasites move from the skin to the liver or other organs after injected by mosquitoes?

Research Project

Project/Area Number 16590344
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionJICHI MEDICAL SCHOOL

Principal Investigator

MATSUOKA Hiroyuki  JICHI MEDICAL SCHOOL, School of Medicine, Professor, 医学部, 教授 (10173816)

Co-Investigator(Kenkyū-buntansha) ARAI Meiji  JICHI MEDICAL SCHOOL, School of Medicine, Lecturer, 医学部, 講師 (30294432)
HIRAI Makoto  JICHI MEDICAL SCHOOL, School of Medicine, Research Associate, 医学部, 助手 (50326849)
Project Period (FY) 2004 – 2005
Keywordsmalaria / transgenic parasite / life cycle / luciferase / luciverin / anopheline mosquito / green fluorescent protein
Research Abstract

To observe how malaria parasites develop in living mosquitoes or mice, we prepared transgenic malaria parasites (Plasmodium berghei). Green fluorescent protein (GFP) gene or luciferase gene were introduced with DHFR-TS (pyrimethamine resistant) gene. Because the GFP transgenic parasites gave fluorescent when they received UV light, we could observe malaria parasites in mosquito midgut and salivary gland. When we used luciferase transgenic parasites, luciferase activity in mice was visualized through the imaging of whole bodies or dissected organs by using an in-vivo Imaging System (IVIS). Oocysts on the midgut and sporozoites in the salivary glands in mosquitoes were visualized with GFP. When we adopted luciferase-transgenic parasites, we obtained a phenomenon that sporozoites stay alive in the skin more than 24 hours after probing by infective mosquitoes. Forty-two hours after the probing, luciferase-transgenic parasites. were developed in the liver. We confirmed in the present study that liver is the specific organ for the malaria parasites for developing in number before invading in the erythrocytes. Furthermore we observed some lymph nodes giving luciferase activity at 42 hours after probing, suggesting that malaria parasites develop in the lymph nodes.

  • Research Products

    (7 results)

All 2006 2005

All Journal Article (7 results)

  • [Journal Article] The effects of blood feeding and exogenous supply of tryptophan on the quantities of xanthurenic acid in the salivary glands of Anopheles stephensi(Diptera : Culicidae)2006

    • Author(s)
      Okech BA, Arai M, Matsuoka H
    • Journal Title

      Biochem Biophys Res Commun 341(4)

      Pages: 1113-1118

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The effects of blood feeding and exogenous supply of tryptophan on the quantities of xanthurenic acid in the salivary glands of Anopheles stephensi (Diptera : Culicidae)2006

    • Author(s)
      Okech BA, Arai M, Matsuoka H
    • Journal Title

      Biochem Biophys Res Commun 341(4)

      Pages: 1113-1118

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Enhanced T-cell response to mosquito extracts by NK cells in hypersensitivity to mosquito bites associated with EBV infection and NK cell lymphocytosis2005

    • Author(s)
      Tokura Y, Matsuoka H, Koga C, Asada H, Seo N, Ishihara S, Adachi A, Ibe M
    • Journal Title

      Cancer Science 96(8)

      Pages: 519-526

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Analysis of T cell receptor(TCR)BV-gene clonotypes in NC/Nga mice developing dermatitis resembling human atopic dermatitis2005

    • Author(s)
      Matsuoka A, Kato T, Soma Y, Takahama H, Nakamura M, Matsuoka H, Mizoguchi M
    • Journal Title

      Journal of Dermatological Science 38(1)

      Pages: 17-24

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Glucose-6-phosphate dehydrogenase(G6PD) mutations in Cambodia : G6PD Viangchan(871G>A) is the most common variant in the Cambodian population.2005

    • Author(s)
      Matsuoka H, Nguon C, Kanbe T, Jalloh A, Sato H, Yoshida S, Hirai H, Arai M, Socheat D, Kawamoto F.
    • Journal Title

      Journal of Human Genetics 50(9)

      Pages: 468-472

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Analysis of T cell receptor (TCR) BV-gene clonotypes in NC/Nga mice developing dermatitis resembling human atopic dermatitis.2005

    • Author(s)
      Matsuoka A, Kato T, Soma Y, Takahama H, Nakamura M, Matsuoka H, Mizoguchi M
    • Journal Title

      Journal of Dermatological Science 38(1)

      Pages: 17-24

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Glucose-6-phosphate dehydrogenase (G6PD) mutations in Cambodia : G6PD Viangchan (871G>A) is the most common variant in the Cambodian population2005

    • Author(s)
      Matsuoka H, Nguon C, Kanbe T, Jalloh A, Sato H, Yoshida S, Hirai H, Arai M, Socheat D, Kawamoto F
    • Journal Title

      Jounal of Human Geneics 50(9)

      Pages: 468-472

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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