2006 Fiscal Year Final Research Report Summary
The molecular mechanism of host responses against cell wall components of mycobacteria
| Project/Area Number |
16590368
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | National Institute of Infectious Diseases (2006)
Osaka City University (2004-2005) |
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Principal Investigator |
KOBAYASHI Kazuo National Institute of Infectious Diseases, Department of Immunology, Director, 免疫部, 部長 (20142432)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Sohkichi Osaka City University Graduate School of Medicine, Department of Host Defense, Associate Professor, 大学院医学研究科, 助教授 (30244073)
FUJIWARA Nagatoshi Osaka City University Graduate School of Medicine, Department of Host Defense, Associate Professor, 大学院医学研究科, 講師 (80326256)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Host response / Tuberculosis / Nontuberculous mycobacteria / Pathogenesis / Serodiagnosis / Vaccine development / Cell wall components / Host defense |
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| Research Abstract |
Mycobacterial diseases, including tuberculosis, leprosy, and disease due to nontuberculous mycobacteria, are the major cause of death from infectious diseases around the world. About one-third of the world population is latently infected with Mycobacterium tuberculosis. Over 8 million new cases and nearly 2 million deaths occur each year. Tuberculosis presents a significant health threat to the world. The pathogenicity of mycobacteria is related to their ability to escape killing by ingested macrophages, latent infection, and induce delayed-type hypersensitivity. This has been attributed to several components of the mycobacterial cell wall, such as surface glycolipids, lipoarabinomannan, complement activation factor, heat-shock protein, and mycobacterial DNA-binding protein. In the present study, we have focused on the molecular mechanism of host responses against cell wall components of mycobacteria, including glycopeptidolipid (GPL) of Mycobacterium avium complex (MAC) and mycobacter
… More
ial DNA-binding protein 1 (MDP1). Serum anti-GPL antibody was detected in patients with MAC diseases, but not in tuberculosis and healthy individuals vaccinated with BCG (sensitivity and specificity 【greater than or equal】 90%). The levels of anti-GPL antibody reflected disease activity. The measurement of antibody to GPL is useful for both diagnosis of MAC disease and assessment of diseases activity. MDP1 participates in both slow growth and adhesion/invasion of mycobacteria into host cells. Treatment of mice with MDP1-DNA complex resulted in host defense against mycobacterial infection by the induction of interferon-g and antibody to MDP1 in the host. Collectively, surface molecules of mycobacteria exert pleiotropic activities in both the microbe and host, and thus participate in the pathogenesis of mycobacterial diseases. The better understanding of molecular pathogenesis in mycobacterial infection may open the new avenue for the development of therapeutic and prophylactic interventions. Less
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Research Products
(35 results)
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[Journal Article] Deletion of kasB in Mycobacterium tuberculosis causes loss of acid-fastness and subclinical latent tuberculosis in immunocompetent mice.2007
Author(s)
Bahatt, A., N.Fujiwara, K.Bhatt, S.S.Gurcha, L.Kremer, B.Chen, J.Chan, S.A.Porcelli, K.Kobayashi, G.S.Besra, W.R.Jacobs, Jr.
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Journal Title
Proc. Natl. Acad. Sci. USA 104
Pages: 5157-5162
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Serological test and chest computed tomography findings in patients with Mycobacterium avium complex lung disease.2007
Author(s)
Kitada, S., Y.Nishiuchi, T.Hiraga, H.Hashimoto, K.Yoshimura, K.Miki, M.Miki, M.Motone, T.Fujikawa, K.Kobayashi, I.Yano, R.Maekura
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Deletion of kasB in Mycobacterium tuberculosis causes loss of acid-fastness and subclinical latent tuberculosis in immunocompetent mice.2007
Author(s)
Bahatt, A., N.Fujiwara, K.Bhatt, S.S.Gurcha, L.Kremer, B.Chen, J.Chan, S.A.Porcelli, K.Kobayashi, G.S.Besra, W.R.Jacobs, Jr
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Journal Title
Proc.Natl.Acad.Sci.USA 104
Pages: 5157-5162
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Serological test and chest computed tomography findings in patients with Mycobacterium avium complex lung disease.2007
Author(s)
Kitada, S, Y.Nishiuchi, T.Hiraga, H.Hashimoto, K.Yoshimura, K.Miki, M.Miki, M.Motone, T.Fujikawa, K.Kobayashi, I.Yano, R.Maekura
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] DNA augments antigenicity of mycobacterial DNA-binding protein 1 and confers protection against Mycobacterium tuberculosis infection in mice.2005
Author(s)
Matsumoto, S., M.Matsumoto, K.Umemori, Y.Ozeki, M.Furugen, T.Tatsuo, Y.Hirayama, S.Yamamoto, T.Yamada, K.Kobayashi
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Journal Title
J. Immunol. 175
Pages: 441-449
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Mycobacterial trehalose 6,6'-dimycolate preferentially induces type 1 helper T cell responses through signal transducer and activator of transcription 4 protein.2005
Author(s)
Oiso, R., N.Fujiwara, H.Yamagami, S.Maeda, S.Matsumoto, S.Nakamura, N.Oshitani, T.Matsumoto, T.Arakawa, K.Kobayashi
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Journal Title
Microb. Pathog. 39
Pages: 35-43
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Mycobacterial trehalose 6,6'-dimycolate preferentially induces type 1 helper T cell responses through signal transducer and activator of transcription 4 protein.2005
Author(s)
Oiso, R, N.Fujiwara, H.Yamagami, S.Maeda, S.Matsumoto, S.Nakamura, N.Oshitani, T.Matsumoto, T.Arakawa, K.Kobayashi
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Journal Title
Microb.Pathog 39
Pages: 35-43
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] DNA augments antigenicity of mycobacterial DNA-binding protein 1 and confers protection against Mycobacterium tuberculosis infection in mice.2005
Author(s)
Matsumoto, S, M.Matsumoto, K.Umemori, Y.Ozeki, M.Furugen, T.Tatsuo, Y.Hirayama, S.Yamamoto, T.Yamada, K.Kobayashi
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Journal Title
J.Immunol 175
Pages: 441-449
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Extracellular mycobacterial DNA binding protein 1 participates in Mycobacterium-lung epithelial cell interaction through hyaluronic acid.2004
Author(s)
Aoki, K., S.Matsumoto, Y.Hirayama, T.Wada, Y.Ozeki, M.Niki, P.Domenech, K.Umemori, S.Yamamoto, A.Mineda, M.Matsumoto, K.Kobayashi
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Journal Title
J. Biol. Chem. 279
Pages: 39798-39806
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Active surveillance of leprosy contacts in country with low prevalence rate.2004
Author(s)
Kai, M., Y.Maeda, S.Maeda, Y.Fukutomi, K.Kobayashi, Y.Kashiwabara, M.Makino, M.A.Abbasi, M.Z.Zubair, P.A.Shah
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Journal Title
Int. J. Lepr. Other Mycobact. Dis. 72
Pages: 50-53
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir.2004
Author(s)
Li, Y., Y.Kawamura, N.Fujiwara, T.Naka, H.Liu, X.Huang, K.Kobayashi, T.Ezaki
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Journal Title
Int. J. Syst. Evol. Microbiol. 54
Pages: 827-835
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Rothia aeria sp.nov., Rhodococcus baikonurensis sp.nov. and Arthrobacter russicus sp.nov., isolated from air in the Russian space laboratory Mir.2004
Author(s)
Li, Y., Y.Kawamura, N.Fujiwara, T.Naka, H.Liu, X.Huang, K.Kobayashi, T.Ezaki
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Journal Title
Int.J.Syst.Evol.Microbiol 54
Pages: 827-835
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Active surveillance of leprosy contacts in country with low prevalence rate.2004
Author(s)
Kai, M., Y.Maeda, S.Maeda, Y.Fukutomi, K.Kobayashi, Y.Kashiwabara, M.Makino, M.A.Abbasi, M.Z.Zubair, P.A.Shah
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Journal Title
Int.J.Lepr.Other Mycobact.Dis. 72
Pages: 50-53
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Extracellular mycobacterial DNA-binding protein 1 participates in Mycobacterium-lung epithelial cell interaction through hyaluronic acid.2004
Author(s)
Aoki, K., S.Matsumoto, Y.Hirayama, T.Wada, Y.Ozeki, M.Niki, P.Domenech, K.Umemori, S.Yamamoto, A.Mineda, M.Matsumoto, K.Kobayashi
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Journal Title
J.Biol.Chem 279
Pages: 39798-39806
Description
「研究成果報告書概要(欧文)」より
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