2005 Fiscal Year Final Research Report Summary
Viral genomic region of Hepatitis C Virus which determine viral replication and drug sensitivity
| Project/Area Number |
16590396
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
MIYAMOTO Michiko Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for Neuroscience, Scientist, 東京都神経科学総合研究所, 研究員 (40190821)
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| Co-Investigator(Kenkyū-buntansha) |
WAKITA Takaji Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for Neuroscience, Senior Research Scientist, 東京都神経科学総合研究所, 副参事研究員 (40280789)
KATO Takanobu Nagoya City University, Graduate School of Medicine, Assistant Professor, 大学院・医学研究科, 助手 (20333370)
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| Project Period (FY) |
2004 – 2005
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| Keywords | HCV / virus / viral genome replication / HCC / chronic hepatitis / interferon |
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| Research Abstract |
Hepatitis C virus (HCV) is a major public health problem, infecting an estimated 170 million people worldwide. Current therapy for HCV-related chronic hepatitis is based on the use of interferon. However, virus clearance rates are insufficient. Investigations to develop the anti-viral therapy or to understand the life cycle of this virus have been hampered by the lack of viral culture systems. We isolated the JFH-1 strain from a patient with fulminant hepatitis, and the JFH-1 subgenomic replicon could replicate efficiently in culture cell without adaptive mutation. Using this JFH-1 strain, analyzed the viral region which is important for the efficient viral replication, viral particle formation and drug resistant. We have found that 5'UTR, NS3 helicase domain and NS5b RNA polymerase regions were important for viral replication. However, other than these region may be important for efficient viral particle formation and secretion. Furthermore, we found that ribavirin and cycrosporin were effective for suppression of viral replication of genotype 2a HCV replicon.
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[Journal Article] Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus.2006
Author(s)
Y Rouille, F Helle, D Deigrange, P Roingeard, C Voisset, E Blanchard, S Belouzard, J McKeating, A Patel, G Maertens, T Wakita, C Wychowski, J Dubuisson
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Journal Title
Journal of Virology (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Detection of Anti-Hepatitis C Virus Effects of Interferon and Ribavirin by a Sensitive Replicon System2005
Author(s)
T Kato, T Date, M Miyamoto, M Sugiyama, Y Tanaka, E Orito, T Ohno, K Sugihara, I Hasegawa, K Fujiwara, K Ito, A Ozasa, M Mizokami, T Wakita.
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Journal Title
Journal of Clinical Microbiology 43
Pages: 5679-5684
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Production of Infectious Hepatitis C Virus in Tissue Culture from a Cloned Viral Genome2005
Author(s)
T Wakita, T Pietschmann, T Kato, T Date, M Miyamoto, Z Zhao, K Murthy, A Habermann, H-G Krausslich, M Mizokami, R Bartenschlager, T J Liang
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Journal Title
Nat Med. 11
Pages: 791-796
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Nuclear Localization of Japanese Encephalitis Virus Core Protein Enhances Viral Replication2005
Author(s)
Y Mori, T Okabayashi, T Yamashita, Z Zhao, T Wakita, K Yasui, F Hasebe, M Tadano, E Konishi, K Moriishi, Y Matsuura.
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Journal Title
J Virol. 79
Pages: 3448-3458
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Regulation of Hepatitis C Virus Replication by Interferon Regulatory Facctor-12004
Author(s)
Kanazawa N, Kurosaki M, Sakamoto N, Enomoto N, Itsui Y, Yamashiro T, Tanabe Y, Maekawa S, Nakagawa M, Chen C-H, Oshima S, Nakamura T, Kato T, Wakita T, Watanabe M
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Journal Title
J Virol 78
Pages: 9713-9720
Description
「研究成果報告書概要(欧文)」より
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