2005 Fiscal Year Final Research Report Summary
Construction and usage of a mouse or human synthetic lymphoid tissue using biocompatible materials.
| Project/Area Number |
16590408
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | National Institute of Biomedical Innovation (2005)
The Institute of Physical and Chemical Research (2004) |
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Principal Investigator |
SUEMATSU Sachiko National Institute of Biomedical Innovation, Laboratory for Immune Cell Regulation, Project Leader, 基盤的研究部免疫細胞制御プロジェクト, プロジェクトリーダー (50250345)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Takeshi RIKEN Research Center for Allergy and Immunology, Immune Surveillance Unit, Unit Leader, 免疫監視機構研究ユニット, ユニットリーダー (40028684)
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| Project Period (FY) |
2004 – 2005
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| Keywords | synthetic lymphoid tissue / stromal cell / biocompatible materials / secondary lymphoid tissue / antigen specific antibody induction / SCID mice / chemokine |
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| Research Abstract |
We demonstrated that a tissue-engineered, lymphoid tissue- like organoid could be constructed by transplantation of stromal cells embedded in biocompatible scaffolds into the renal subcapsular space in mice. This organoid had a tissue structure similar to secondary lymphoid organs, which contained compartmentalized B- cell and T-cell clusters, high endothelial venule-like vessels, germinal centers and follicular dendritic cell networks. Furthermore, the organoid was transplantable to naive normal or severe combined immunodeficiency (SCID) mice, and antigen-specific, IgG-isotype antibody formation could be induced soon after intravenous administration of the antigen. This simplified system of lymphoid tissue・like organoid construction will facilitate analyses of cell-cell interactions required for development of secondary lymphoid organs and efficient induction of adaptive immune responses, and may have possible applications in the treatment of immune deficiency.
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