2006 Fiscal Year Final Research Report Summary
Studies on prognostic diagnostics in rupture of atherosclerotic plaque
| Project/Area Number |
16590463
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
KANOH Yuhsaku Kitasato University, School of Medicine, Assistant Professor, 医学部, 講師 (50245388)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Laboratory Medicine / Pathology / Preventive Medicine / Internal Medicine / Cardiology・Hypertension |
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| Research Abstract |
1) Analysis of plaque of atherosclerosis by ultrasonography
We checked the ultrasonography of bilateral common and internal carotid artery for 2'000 cases that have risk factors (diabetes mellitus, hypertension, hyperlipidemia, etc) of atherosclerosis at Kitasato university hospital. The plaque of atherosclerosis turned worse in order of echolucent type, echogenic type, calcification type, ulcer type with progression of arteriosclerosis. Therefore, the degree of arteriosclerosis was high in complication of diabetes mellitus and hypertension.
2) Measurement of brachial-ankle PWV (ba PWV)
The ba PWV that reflects the degree of arteriosclerosis was highest in case of hypertension. The complication of diabetes mellitus and hypertension tended to increase, and it was thought that their risk factors strongly affected the progression of arteriosclerosis.
3) Measurement of lipid and inflammatory markers in serum
Total cholesterol/HDL-cholesterol (T.cho/HDL-cho) ratio, LDL-cholesterol/HDL-cholesterol (LDL-cho/HDL-cho) ratio, Malondialdehyde-LDL/LDL-cholesterol (MDA-LDL/LDL-cho) ratio, hypersensitivity CRP (hs CRP) increased with progression of arteriosclerosis. Levels of these markers were significantly higher than those of normal controls. Therefore, it was thought that these markers reflected the change of early stage of arteriosclerosis
4) Analysis of the gene of MMP-2 and MMP-9
We analyzed the genome DNA of MMP-2 and MMP-9 in patients with atherosclerosis of common and internal carotid artery.
The DNA mutation of MMP-2 and MMP-9 were observed in peripheral blood specimens obtained from patients with atherosclerosis of common and internal carotid artery. A genetic polymorphism of MMP-2 and MMP-9 may be linked to progression of atherosclerosis of common and internal carotid artery.
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