2005 Fiscal Year Final Research Report Summary
Genetic typing and clinical method of detecting metallo-beta-lactamase-producers in gram-negative rods in Japan
| Project/Area Number |
16590470
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | National Institute of Infectious Diseases, Japan |
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Principal Investigator |
SHIBATA Naohiro National Institute of Infectious Diseases, Japan, Bacterial Infection Control and Pathogenesis, chief researcher, 細菌第二部, 研究員 (50311402)
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| Project Period (FY) |
2004 – 2005
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| Keywords | metallo-beta-lactamase / genetic typing / drug-resistance / gram-negative bacteria |
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| Research Abstract |
Since metallo-beta-lactamase (MBLs) can hydrolyze a very wide range of broad-spectrum beta-lactams, MBL-producing gram-negative bacteria usually demonstrate consistent resistance to a variety of broad-spectrum beta-lactams, including oxyiminocephalosporins, cephamycins, and carbapenems, which are the least resort for control of infections caused by gram-negative bacteria. Thus, MBL-producing gram-negative bacteria have been recognized to be among the most important nosocomial pathogens, and further proliferation of these strains in clinical settings will pose a serious global problem in the future. The worldwide spread of this kind of organism is becoming a general concern, since several MBL-producing gram-negative bacteria have recently been reported outside Japan. On the other hand, the emergence of gram-negative bacterial species with acquired resistance to various broad-spectrum beta-lactams is becoming a worldwide clinical problem. Srains producing TEM- or SHV-derived extended-spe
… More
ctrum beta-lactamases (ESBLs) usually demonstrate high-level resistance to broad-spectrum oxyimino beta-lactams such as ceftiazidime and cefotaxime. Moreover, several K.pneumoniae strains that showed resistance to cephamycins as well as oxyimino cephalosporins were also found to priduce AmpC-type beta-lactamases. Indeed, PCR analyses usually give reliable and satisfactory results, but this method is of limited practical use for daily application in clinical laboratories because of the cost. We demonstrated a convenient test (SMA test) by using thiol compound in 2000. But this method was only MBL detection. In this study, we tried to screen beta-lactamase producers by using disk diffusion test with the combination of SMA and various antibiotic disks. By this method, CAZ-resistant strains producing AmpC or ESBLs were distinguishable from MBL producers. Therefore the method described in this study is very helpful for screening MBL- and other beta-lactamase-producing strains in dauly clinical labolatory testing. Less
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[Journal Article] PCR Classification of CTX-M-Type Beta-Lactamase Genes Identified in Clinically Isolated Gram-Negative Bacilli in Japan.2006
Author(s)
Shibata N, H Kurokawa, Y Doi, T Yagi, K Yamane, J Wachino, S Satowa, K Kimura, S Ishikawa, H Kato, Y Ozawa, K Shibayama, K Kai, F Konda, Y Arakawa.
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Journal Title
Antimicrobial Agents and Chemotherapy 50(2)
Pages: 791-795
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Novel plasmid-Mediated 16S rRNA Methilase, RmtC, found in a p roteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides.2006
Author(s)
Wachino J, K Yamane, K Shibayama, H Kurokawa, N Shibata, S Suzuki, Y Doi, K Kimura, Y Ike, Y Arakawa
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Journal Title
Antimicrobial Agents and Chemotherapy 50(1)
Pages: 178-184
Description
「研究成果報告書概要(欧文)」より
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