| Research Abstract |
In this study, mitochondrial DNA haplogrouping by SNaPshot-single nucleotide extension reaction, TaqMan PCR method, and suspension array technology had been carried out.
In SNaPshot-single nucleotide extension reaction, to detect the 3172^<nd>, 3206^<th>, 3338^<th>, 3394^<th>, 4715^<th>, 4761^<st>, 4793^<rd>, 4833^<rd>, 5147^<th>, 9824^<th>, 9923^<rd>, 10310^<th>, 10321^<st>, 10345^<th>, 10373^<rd>, 10397^<th> and 10410^<th> single nucleotide polymorphisms of mitochondrial DNA, primers were designed, and then, multiplex detection system was developed.
In TaqMan PCR method, TaqMan probes and primers for the 10398^<th> and 10400^<th> nucleotide to distinguish between haplogroup M and N, 4833^<rd> to detect haplogroup G, 5178^<th> to detect D, 15487^<th> to detect M8a・C4・CZ, 6455^<th> to detect M7 were designed. Rapidly detection of these 6 SNPs was developed.
Luminex system-suspension array technology was carried out for mitochondrial DNA screening. In this study, IB, IC1, IC2 and ID in HV I, IIA1, IIA2, IIB and IIC were analyzed. In 105 unrelated Japanese, 19 SNPs were detected, and 50 kinds of mitochondrial DNA were detected.
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