2006 Fiscal Year Final Research Report Summary
The effects of lectins on spontaneous colitis in HLA B27 transgenic rats
| Project/Area Number |
16590588
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
SASAKI Masaya Shiga University of Medical Science, University Hospital, Associate Professor, 医学部, 講師 (40242979)
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| Project Period (FY) |
2004 – 2006
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| Keywords | HLA-B27 transgenic rat / lectins / DSS colitis / curcumin |
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| Research Abstract |
Lecints have significant proliferative effects on the intestine and have some potential therapeutic effects on the intestinal injury, such as indomethacine induced small intestinal ulceration. HLA-B27 transgenic rats expressing human leukocyte antigen (HLA)-B27 and human β 2-microglubulin spontaneously develop chronic colitis, which resembles human inflammatory bowel disease. In this study, I examined the efficacy of lectins such as phytohemagglutuin (PHA) and concanavalian-A (Con-A) against spontaneous colitis in HLA B27 rats. I also examined the effects of curcumin against this spontaneous colitis.
The HLA B27 rats were divided into four groups after development of colitis, PHA group, Con-A group, curcumin group and control group. After 14 days, we examined the body weight changes, wet weights and lengths of the colon, mucosal myloperoxidase activities, serum IL-6 levels and histological damage score. There are no significant differences in histological damage score, mucosal myeloperoxidase activity and serum IL-6 levels. Oral administration of PHA or Con-A did not suppress the spontaneous colitis that developed in HLA-B27 rats. Curcumin slightly suppressed this spontaneous colitis.
I also examined the therapeutic effects of lectins and curcumin against mice DSS induced colitis. In this model, PHA and Con-A did not suppress the colitis. However, curcumin has significant therapeutic effects on DSS colitis
In summary, oral administration of lectins did not show the significant therapeutic effects against colitis, but curcumin has a potential therapeutic effects on the colitis.
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