2005 Fiscal Year Final Research Report Summary
Development of New device for Regeneration of small intestinal tissue
| Project/Area Number |
16590590
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto University |
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Principal Investigator |
NAKASE Hiroshi Kyoto University, Department of Gastroenterology & Hepatology, Assistant Professor, 医学研究科, 講師 (60362498)
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| Co-Investigator(Kenkyū-buntansha) |
TABATA Yasuhiko Kyoto University, Institute for Frontier Medical Science, Professor, 再生医科学研究所, 教授 (50211371)
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| Project Period (FY) |
2004 – 2005
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| Keywords | small intestine regenerating tube / bone marrow cell / basic FGF |
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| Research Abstract |
1. Development of innovative polylactic acid tube (or polyglycolic acid) for regenerating small intestinal tissue. We have completed innovative polylactic acid (or polyglycolic acid) tube which could be suitable for regeneration of small intestine. We also showed that bFGF is most useful growth factor for regeneration of small intestinal tissue. Therefore, we completed the biodegradable gelatin microspheres, which can continuously release bFGF. We attached these microspheres to inside of the tube and injected bone marrow cells into this tube (we named it as small intestinal regenerating tube=SIR tube.). We transplanted SIR tube to small intestine of rat, which had taken resection of small intestine for experiment. Interestingly, regeneration of small intestinal tissue was observed inside of SIR tube 2 months after transplanting.
2. We checked regenerated small intestinal tissue histologically and functionally. As a result, minute endoscopic findings revealed that villous epithelium was appeared one month after transplanting.
3. Moreover, we investigated the proportion of immune-regulating cells in regenerated small intestine. Histological and FACS analysis revealed that there was no significant difference of the proportion of macrophages, T, and B cells in between regenerated and normal small intestine.
4. We injected GFP bone marrow cells in SIR tube. Three months after transplanting this tube, we investigated what kinds of cell GFP bone marrow cells would differentiate into in regenerated small intestine. As a result, confocal microscopic findings showed that GFP was positive for myofibroblasts and small intestinal epithelium, which suggested that transplanted bone marrow cells differentiated into epithelium and mesenchymal cells and compose of newly regenerated small intestine.
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