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2005 Fiscal Year Final Research Report Summary

Mechanism for the involvement of the endothelin system in craniofacial and cardiovascular development

Research Project

Project/Area Number 16590659
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionThe University of Tokyo

Principal Investigator

KURIHARA Yukiko  The University of Tokyo, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (80345040)

Co-Investigator(Kenkyū-buntansha) KURIHARA Hiroki  The University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20221947)
AMANO Tomokazu  The University of Tokyo, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 寄付講座教員(助手相当) (50359634)
Project Period (FY) 2004 – 2005
KeywordsNeural crest cell / cardiovascular development / endothelin / endothelin A receptor
Research Abstract

We have demonstrated that the ET-1(endothelin-1) signal from branchial epithelium to mesenchyme is important to act on the ETAR(ETA receptor) expressed in the neural crest cells. This pathway regulates Dlx5/6 as homeotic gene which is down-steam of its pathway, and plays a role of dorso-ventral patterning in the 1st branchial arch. In other words, we have demonstrated that both dorsal and ventral 1st branchial arch is determined to be upper jaw pattern at the midgestation embryo, but only the ventral portion receives the ET-1-ETAR-Dlx5/6 signal to specify the lower pattern. Then we made the mice in which transgene can be knocked-in into the ETAR gene locus. For this purpose, mutant lox sequences were inserted into the ETAR gene locus to allow Cre recombinase-mediated cassette exchange. The phenotype of ETAR-mutant lox homozygotes are the same as the phenotype of ET-1 or ETAR homozygotes. We then knocked in the LacZ gene into the downstream of ETAR promoter in these mice. Consequently, target cells of the ET-1 signal contributed to branchial arch and cardiovascular development were visualized. These mice enable us to trace the process of cardiovascular development and to analyze of mutual interaction between neural crest cells and other cells. Further knock-in studies are expected to reveal the molecular mechanism underlying branchial arch and cardiovascular development.

  • Research Products

    (8 results)

All 2006 2005 2004

All Journal Article (8 results)

  • [Journal Article] Transcriptional activity of Pax3 is co-activated by TAZ.2006

    • Author(s)
      Murakami M
    • Journal Title

      Biochem. Biophys. Res. Commun. 339

      Pages: 533-539

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transcriptional activity of Pax3 is co-activated by TAZ.2006

    • Author(s)
      Murakami M
    • Journal Title

      Biochem.Biophys.Res.Commun. 339

      Pages: 533-539

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Idl gene transfer confers angiogenic property on fully differentiated endothelial cells and contributes to therapeutic angiogenesis.2005

    • Author(s)
      Nishiyama K
    • Journal Title

      Circulation 112

      Pages: 2840-2850

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Id1 gene transfer confers angiogenic property on fully differentiated endothelial cells and contributes to therapeutic angiogenesis.2005

    • Author(s)
      Nishiyama K
    • Journal Title

      Circulation 112

      Pages: 2840-2850

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Endothelin-1 regulates cardiac sympathetic innervation in the rodent heart by controlling nerve growth factor expression.2004

    • Author(s)
      Ieda M
    • Journal Title

      J. Clin. Invest. 113

      Pages: 876-884

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Endothelin-1 regulates the dorsoventral branchial arch patterning in mice.2004

    • Author(s)
      Ozeki H
    • Journal Title

      Mech.Dev. 121

      Pages: 387-395

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Temporal requirement of signaling cascade involving endothelin-1/endothelin A receptor in branchial arch development.2004

    • Author(s)
      Fukuhara S
    • Journal Title

      Mech.Dev. 121

      Pages: 1223-1233

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Endothelin-1 regulates cardiac sympathetic innervation in the rodent heart by controlling nerve growth factor expression.2004

    • Author(s)
      Ieda M
    • Journal Title

      J.Clin.Invest. 113

      Pages: 876-884

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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