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2005 Fiscal Year Final Research Report Summary

The identification of disease-specific proteins in urine by proteomics and its clinical application

Research Project

Project/Area Number 16590781
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionNiigata University

Principal Investigator

SAKATSUME Minoru  Niigata University, Medical and Dental Hospital, Assistant, 医歯学総合病院, 助手 (70334662)

Project Period (FY) 2004 – 2005
KeywordsUrinary protein / Proteomics / Urinary immune cells / MALDI-TOF MS / Mass spectrometry
Research Abstract

The purpose of this research is to establish the new non-invasive diagnostic method of kidney diseases by urine sample. The research products of this fiscal year are ; 1) The classifications of kidney diseases into 5 types : kidney diseases was classified by data obtained form analyses of urine cells (the morphology of erythrocytes and leukocyte-surface markers with the flow cytometer), electrophoretic pattern of urine protein visualized with a high sensitivity detection system and the findings of kidney biopsy. Patent application was carried out for a part of this study. 2) The analysis of urine protein by proteomics : disease-specific pattern was found in alpha1 fraction, and the pattern was examined in relevance with the protein-degrading enzyme in urine (Machii R). The content of dominant bands was analyzed using the MALDI-TOF mass spectrometer. 3) Comprehensive gene profiling of glomerulonephritis model and the identification of nephritis-specific genes through DNA chip analysis : … More three nephritis-specific genes were discovered. The increased gene expression was checked by Northern blot, and the localization of genes and proteins was investigated by in situ hybridization and immunohistochemistry. Moreover, it is under investigation whether they were applicable as a diagnostic marker of glomerulonephritis. To determine whether, the sensitivity and the specificity were also examined (Ogawa A, Tsubata Y). One discovered gene could be considered as an inflammation factor (metalloprotease-12 ; MMP-12). It is now under investigation whether it is detected in urine of patients with glomerulonephritis, and whether it is possible to apply this as a specific nephritis marker in urine. On the other hand, the suppressive factors for MMP-12 expression were screened, and fibrate, a PPARa-ligand, was found as a therapeutic agent for glomerulonephritis (Saga D). Thus, while some studies have not been completed, a wide range of results such as the discovery of biomarkers and the establishment of marker for screening of therapeutic reagents was obtained from this project. Less

  • Research Products

    (14 results)

All 2006 2005 2004

All Journal Article (12 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Examination of the molecular diversity of alpha(1) antitrypsin in urine : Deficit of an alpha(1) globulin fraction on cellulose acetate membrane electrophoresis.2005

    • Author(s)
      Machii, R
    • Journal Title

      J Clin Lab Anal 19(1)

      Pages: 16-21

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Bezafibrate markedly suppresses rat anti-glomerular membrane nephritis.2005

    • Author(s)
      Saga, D
    • Journal Title

      Kidney Int 67(5)

      Pages: 1821-1829

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 尿蛋白のプロテオミクス2005

    • Author(s)
      坂爪 実
    • Journal Title

      腎と透析 58(3)

      Pages: 365-369

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 早期診断と臨床検査:早期診断に有用な検査とその利用法腎・泌尿器疾患2005

    • Author(s)
      坂爪 実
    • Journal Title

      日本内科学会雑誌 94(12)

      Pages: 65-71

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The Analysis of the Allograft Inflammatory Factor-1 Expression in Rat Anti-Glomerular Basement Membrane Nephritis.2005

    • Author(s)
      Tsubata Y
    • Journal Title

      J Am Soc Nephrol 16

      Pages: 641A

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A Smooth Muscle Cell Marker, SM22, is Expressed in Severely Injured Podocytes in Rat Anti-Glomerular Basement Membrane Nephritis.2005

    • Author(s)
      Ogawa, A
    • Journal Title

      J Am Soc Nephrol 16

      Pages: 646A

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Examination of the molecular diversity of alpha(1) antitrypsin in urine : Deficit of an alpha(1) globulin fraction on cellulose acetate membrane electrophoresis.2005

    • Author(s)
      Machii R, Sakatsume M, Kubota R, Kobayashi S, Gejyo F, Shiba K
    • Journal Title

      J Clin Lab Anal 19(1)

      Pages: 16-21

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Bezafibrate suppresses rat anti-glomerular basement membrane crescentic glomerulonephritis.2005

    • Author(s)
      Saga D, Sakatsume M, Ogawa A, Tsubata Y, Kaneko Y, Kuroda T, Sato F, Ajiro J, Kondo D, Miida T, Narita I, Gejyo F.
    • Journal Title

      Kidney Int 67(5)

      Pages: 1821-1829

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The analysis of urinary proteins by proteomics.2005

    • Author(s)
      Sakatsume M, Narita I, Gejyo F, Saito A
    • Journal Title

      Kidney and Dialysis (Japanese) 58(3)

      Pages: 365-369

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Early diagnosis by laboratory medicine in Nephrology and Urology.2005

    • Author(s)
      Sakatsume M, Gejyo F
    • Journal Title

      J Jpn Soc Int Medicine (Japanese) 94(12)

      Pages: 65-71

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The Analysis of the Allograft Inflammatory Factor-1 Expression in Rat Anti-Glomerular Basement Membrane Nephritis.2005

    • Author(s)
      Tsubata Y, Sakatsume M, Ogawa A, Saga D, Narita I, Gejyo F
    • Journal Title

      J Am Soc Nephrol 16(Abstract)

      Pages: 641A

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Smooth Muscle Cell Marker, SM22, Is Expressed in Severely Injured Podocytes in Rat Anti-Glomerular Basement Membrane Nephritis.2005

    • Author(s)
      Ogawa A, Sakatsume M, Tsubata Y, Saga D, Narita I, Gejyo F
    • Journal Title

      J Am Soc Nephrol 16(Abstract)

      Pages: 646A

    • Description
      「研究成果報告書概要(欧文)」より
  • [Patent(Industrial Property Rights)] 腎疾患の活動性判定法およびその装置2006

    • Inventor(s)
      坂爪 実ほか
    • Industrial Property Rights Holder
      国立大学法人新潟大学
    • Industrial Property Number
      国際出願PTC/JP2006/303676
    • Filing Date
      2006-03-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 腎疾患の病態分類法2004

    • Inventor(s)
      坂爪 実ほか
    • Industrial Property Rights Holder
      国立大学法人新潟大学
    • Industrial Property Number
      特願2004-310144
    • Filing Date
      2004-10-25
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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