2005 Fiscal Year Final Research Report Summary
Epileptogenicity of neocortex in the novel rat model of focal cortical dysplasia
| Project/Area Number |
16590832
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | KYUSHU UNIVERCITY |
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Principal Investigator |
SHIGETO Hiroshi Kyushu University Hospital, Research Associate, 大学病院, 助手 (50335965)
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| Co-Investigator(Kenkyū-buntansha) |
OHYAGHI Yasumasa Kyushu University Hospital, Assistant Professor, 大学病院, 講師 (30301336)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Epilepsy / Focal cortical malformation / Neuronal migration disorder / Animal model / Kindling |
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| Research Abstract |
Focal cortical malformation is an important cause of intractable epilepsy in human. However, its in vivo epileptogenicity remains unclear. Freeze lesions postnatally introduced in the hindbrain of rodents produce extra-sulci and microgiri demonstrating only in vitro epileptogenicity. We, therefore, aimed to develop a prenatally introduced freeze lesion model and prove its in vivo epileptogenicity.
At 18 days post-conception, a metal probe cooled by liquid nitrogen was placed on the left scalp of a Sprague-Dawley rat embryo, through the uterus wall. After birth, electrical kindling stimulation was applied on between the right fronto-medial and fronto-lateral points on the frontal lobe of rat pups, and threshold and duration of afterdischarges (ADs) were measured from 0 to 40 days. Brain tissues from electrically stimulated and unstimulated animals were immunohistochemically examined.
The freeze lesion rats developed significantly longer ADs in the cortex and hippocampus compared to the sham operated and control animals, and acquired hippocampal kindling earlier after electrical stimulation although spontaneous seizure was not revealed. Brains from freeze lesioned rats displayed severe disorganization of cortical layers with ramdamly oriented dendrites and axons and nodular heterotopia in the cortical layer and/or the subcortical white matter. Immunoreactivities for N-methyl-D-aspartate receptor subunits 1, 2B and glutamate/aspartate transporter stainings were significantly enhanced by the semi-quantitative densitometry in the lesion, which was markedly potentiated by electrical stimulation.
Freeze lesion created during the late embryonic stage induces focal cortical malformation with neuronal migration abnormality, which exhibits in vivo epileptogenicity on electrical stimulation.
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[Journal Article] Functional network of the basal ganglia and cerebellar motor loops in vivo : different activation patterns between self-initiated and externally triggered movements.2006
Author(s)
Taniwaki T, Okayama A, Yoshiura T, Togao O, Nakamura Y, Yamasaki T, Ogata K, Shigeto H, Ohyagi Y, Kira J, Tobimatsu S.
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Journal Title
Neuroimage 31
Pages: 745-53
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Functional network of the basal ganglia and cerebellar motor loops in vivo : different activation patterns between self-initiated and externally triggered movements.2006
Author(s)
Taniwaki T, Okayama A, Yoshiura T, Togao 0, Nakamura Y, Yamasaki T, Ogata K, Shigeto H, Ohyagi Y, Kira J, Tobimatsu S.
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Journal Title
Neuroimage. 31
Pages: 745-53
Description
「研究成果報告書概要(欧文)」より
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