• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

The role of genes of forkhead transcription factors in type2 diabetes and obesity.

Research Project

Project/Area Number 16590862
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionTohoku University

Principal Investigator

HINOKIO Yoshinori  Tohoku University, Hospital, Lecturer, 病院, 講師 (10282071)

Co-Investigator(Kenkyū-buntansha) SUZUKI Susumu  Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (70216399)
KATAGIRI Hideki  Tohoku University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (00344664)
ISHIHARA Hisamitsu  Tohoku University, Hospital, Research Associate, 病院・助手 (60361086)
Project Period (FY) 2004 – 2005
Keywordstype 2 diabetes / transcription factor / oxidative stress
Research Abstract

With DNA extracted from blood samples of unrelated Japanese type 1 and type 2 diabetic patients and non-diabetic controls, we screened mutations and polymorphisms with direct sequencing in the Foxa-2 gene. The screening revealed four polymorphisms of Foxa-2 gene. We have found that two alleles among the polymorphisms in Foxa-2 gene. The frequencies of A alleles of two polymorphisms, nt 537 and codon279, in the gene were significantly higher than those of the opposite allele (p<0.05). From these facts, it is suggested that the polymorphisms in Foxa-2 gene are involved with clinical characteristics of early-onset diabetes.
With DNA extracted from blood samples of 96 Japanese type2 diabetic patients and 102 non-diabetic controls, we screened mutations and polymorphisms with direct sequencing in heme oxygenase (HO-1) gene. With the screening of HO-1 gene, we identified three polymorphisms, SNPHO1(A/T), SNPHO2(A/G) and (GT)n repeat in the gene. The allele frequencies SNPHO1 and ANPHO2 were n … More ot significantly different between diabetic group and control group. We determined the number of GT repeats in the HO-1 promoter in diabetics and controls and compared the frequencies of short repeat (class S) and long repeat (class L) alleles. In non-diabetic group, the number of patients with hyperlipidemia was significantly larger in class L than in class S (Chi-square test p<0.05). Both the systolic and diastolic pressures in diabetic group were significantly higher in class L than in class S (p<0.05). In non-diabetic group, the number of patients with hyperlipidemia was significantly larger in the case with A/A allele in the SNPHO1 than in the case with A/T allele or T/T allele (Chi-square test p<0.05). Both the systolic and diastolic pressures in diabetic group were significantly higher in the case with A/A allele in SNPHO1 than in with A/T allele or T/T allele.
Taken together, these data may suggest a role of polymorphisms of Foxa-2 gene and HO-1 gene in diabetes, hyperlipidemia and hypertension. That requires further investigation to test these hypotheses. Less

  • Research Products

    (6 results)

All 2005

All Journal Article (6 results)

  • [Journal Article] Secondary sulfonylurea failure : comparison of period until insulin treatment between diabetic patients treated with gliclazide and glibenclamide.2005

    • Author(s)
      Satoh J, Takahashi K, Takizawa Y, Ishihara H, Hirai M, Katagiri H, Hinokio Y, Suzuki S, Tsuji I, Oka Y.
    • Journal Title

      Diabetes Res Clin Pract 70

      Pages: 291-297

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] WRN gene 1367 Arg allele protects against development of type 2 diabetes mellitus.2005

    • Author(s)
      Hirai M, Suzuki S, Hinokio Y, Yamada T, Yoshizumi S, Suzuki C, Satoh J, Oka Y.
    • Journal Title

      Diabetes Res Clin Pract 69

      Pages: 287-292

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic variations of urotensin II gene in type 2 diabetes2005

    • Author(s)
      Hinokio Y, Suzuki S, Oka Y.
    • Journal Title

      Nippon Rinsho 63

      Pages: 193-197

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Secondary sulfonylurea failure : comparison of period until insulin treatment between diabetic patients treated with gliclazide and glibenclamide.2005

    • Author(s)
      Satoh J, Takahashi K, Takizawa Y, Ishihara H, Hirai M, Katagiri H, Hinokio Y, Suzuki S, Tsuji I, Oka Y.
    • Journal Title

      Diabetes Res Clin Pract. 70

      Pages: 291-297

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] WRN gene 1367 Arg allele protects against development of type 2 diabetes mellitus.2005

    • Author(s)
      Hirai M, Suzuki S, Hinokio Y, Yamada T, Yoshizumi S, Suzuki C, Satoh J, Oka Y.
    • Journal Title

      Diabetes Res Clin Pract. 69

      Pages: 287-292

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genetic variations of urotensin II gene in type 2 diabetes2005

    • Author(s)
      Hinokio Y, Suzuki S, Oka Y.
    • Journal Title

      Nippon Rinsho. 63

      Pages: 193-197

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi