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2005 Fiscal Year Final Research Report Summary

Anobel apolipoprotein receptor : ApoB48 receptor

Research Project

Project/Area Number 16590870
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionTokyo Medical and Dental University Graduate School

Principal Investigator

SHIMOKADO Kentaro  Tokyo Medical and Dental University Graduate School, 大学院・医歯学総合研究科, Professor (30192115)

Co-Investigator(Kenkyū-buntansha) TANAKA Akira  Kanto Gakuin Univesity, 人間環境学部, Professor (70171733)
YUI Masakatu  Tokyo Medical and Dental University, Assistant Professor (70323714)
Project Period (FY) 2004 – 2005
Keywordsdyslipidemia / postprandial hyperlipidemia / lipoprotein receptor / differentiation / macrophage / T lymphocyte / B lymphocyte
Research Abstract

ApoB48 receptor (ApoB48R) is a new lipoprotein receptor found on monocytes-macrophages, but not much is known about the regulation of expression of this receptor. We studied the distribution of this receptor among peripheral blood mononuclear cells, and apoB48R expression during monocyte differentiation.Human monocytes and lymphocytes were separated from peripheral blood by magnetic beads conjugated with anti-CD14, anti-CD3-, and anti-CD19 antibody. Umbilical cord blood stem cells were separated by anti-CD34. Peripheral blood monocytes, T lymphocytes and B lymphocytes all expressed apoB48R similarly. Contrary, CD34 positive precursor cells only weakly expressed the receptor. Human monocytic cell lines U937 cells were differentiated to monocyte/macrophages with phorbol 12-myristate 13-acetate (PMA). The amount of mRNA for apoB48R increased 4 times after 72 hours of PMA stimulation in this cell line. ApoB48R protein also increased as U937 differentiated. The expression of apoB48R did not change during the differentiation from circulating monocytes to adherent macrophages. Both peripheral blood monocytes and lymphocytes express apoB48R The expression increases during the differentiation from precursor cells to nonocytes, but not during the differentiation from circulating monocytes to macrophages.

  • Research Products

    (12 results)

All 2008 2007 2006 2005

All Journal Article (11 results) Presentation (1 results)

  • [Journal Article] Hyperbaric oxygen induces basic fibroblast growth factor and hepatocyte growth factor expression, and enhances blood perfusion and muscle regeneration in mouse ischemic hind limbs2007

    • Author(s)
      Asano T., Kaneko E., Shinozaki S., Imai Y, Shibayama M., Chiba T., Ai M., Kawakami A., Asaoka H., Nakayama T., Mano Y., Shimokado K.
    • Journal Title

      Circ J 71

      Pages: 405-411

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Site-specific effect of estradiol on gene expression in the adipose tissue of ob/ob mice2007

    • Author(s)
      Shinozaki S., Chiba T., Kokame K., Miyata T., Kawakami A., Kaneko E., Yoshida M., Shimokado K.
    • Journal Title

      Horm Metab Res 39

      Pages: 192-196

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Selective NFkappaB Inhibitor, DHMEQ, Reduced Atherosclerosis in ApoE-deficient mice2006

    • Author(s)
      Chiba T, Kondo Y, Shinozaki S, Kaneko E, Ishigami A, Maruyama N, Umezawa K, Shimokado K.
    • Journal Title

      J Atheroscler Thromb. 6

      Pages: 308-313

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Favorable effects of early insulin secretion by nateglinide on postprandial hyperlipidemia in patients with type 2 diabetes2006

    • Author(s)
      Ai M, Tanaka A, Ogita K, Shimokado K.
    • Journal Title

      Diabetes Care. 5

      Pages: 1180

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Metabolic syndrome and the management of traditional risk factors for ischemic heart disease in elderly people2005

    • Author(s)
      Shimokado K.
    • Journal Title

      Nippon Ronen Igakkai Zasshi. 42

      Pages: 508-11

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Danshen inhibits oxystero1-induced endothelial cell apoptosis in vivo.2005

    • Author(s)
      Nakazawa T, Xui N, Hesong Z, Kinoshita M, Chiba T, Kaneko E, Yui K, Shimokado K.
    • Journal Title

      J Atheroscler Thromb. 12

      Pages: 132-7

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Insulin signaling in arteries prevents smooth muscle apoptosis.2005

    • Author(s)
      Nakazawa T, Chiba T, Kaneko E, Yui K, Yoshida M, Shimokado K.
    • Journal Title

      Arterioscler Thromb Vasc Biol. 25

      Pages: 760-5

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pitavastatin inhibits remnant lipoprotein-induced macrophage foam cell formation through ApoB48 receptor-dependent mechanism.2005

    • Author(s)
      Kawakami A, Tani M, Chiba T, Yui K, Shinozaki S, Nakajima K, Tanaka A, Shimokado K, Yoshida M.
    • Journal Title

      Arterioscler Thromb Vasc Biol. 25

      Pages: 424-9

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pitavastatin inhibits remnant lipoprotein-induced macrophage foam cell formation through ApoB48 receptor-dependent mechanism2005

    • Author(s)
      Kawakami A, Tani M, Chiba T, Yui K, Shinozaki S, Nakajima K, Tanaka A, Shimokado K, Yoshida M.
    • Journal Title

      Arterioscler Thromb Vasc Biol. 25

      Pages: 424-429

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Insulin Signaling in Arteries Prevents Smooth Muscle Apoptosis2005

    • Author(s)
      Nakazawa T, Chiba T, Kaneko E, Yui K, Yoshida M, Shimokado K.
    • Journal Title

      Arterioscler Thromb Vasc Biol. 25

      Pages: 760-765

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Radical scavengers inhibit oxysterol-induced endothelial apoptosis in vivo2005

    • Author(s)
      Nakazawa, T., Xui Ning, Zeng Hesong, Kinoshita, M., Chiba, T., Kaneko, E, Yui, K., Shimokado, K
    • Journal Title

      J Arterioscer Thromb 12

      Pages: 132-137

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The expression of apo48 receptor increases the uptake of remnant lipoprotein by cultured hepatocytes2008

    • Author(s)
      Takai M, Shinozaki S, Ishi H, Chiba T, Kawakami A, Yoshida M, Tanaka A, Shimokado K.
    • Organizer
      6^<th> Congress of the Asian Pacific Society of Atherosclerosis and Vascular Diseases
    • Place of Presentation
      Hong Kong
    • Year and Date
      2008-09-26
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2011-06-18  

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