Anobel apolipoprotein receptor : ApoB48 receptor

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 16590870
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Tokyo Medical and Dental University Graduate School
• Principal Investigator: SHIMOKADO Kentaro Tokyo Medical and Dental University Graduate School, 大学院・医歯学総合研究科, Professor (30192115)
• Co-Investigator(Kenkyū-buntansha): TANAKA Akira Kanto Gakuin Univesity, 人間環境学部, Professor (70171733) ; YUI Masakatu Tokyo Medical and Dental University, Assistant Professor (70323714)
• Project Period (FY): 2004 – 2005
• Keywords: dyslipidemia / postprandial hyperlipidemia / lipoprotein receptor / differentiation / macrophage / T lymphocyte / B lymphocyte

Research Abstract

ApoB48 receptor (ApoB48R) is a new lipoprotein receptor found on monocytes-macrophages, but not much is known about the regulation of expression of this receptor. We studied the distribution of this receptor among peripheral blood mononuclear cells, and apoB48R expression during monocyte differentiation.Human monocytes and lymphocytes were separated from peripheral blood by magnetic beads conjugated with anti-CD14, anti-CD3-, and anti-CD19 antibody. Umbilical cord blood stem cells were separated by anti-CD34. Peripheral blood monocytes, T lymphocytes and B lymphocytes all expressed apoB48R similarly. Contrary, CD34 positive precursor cells only weakly expressed the receptor. Human monocytic cell lines U937 cells were differentiated to monocyte/macrophages with phorbol 12-myristate 13-acetate (PMA). The amount of mRNA for apoB48R increased 4 times after 72 hours of PMA stimulation in this cell line. ApoB48R protein also increased as U937 differentiated. The expression of apoB48R did not change during the differentiation from circulating monocytes to adherent macrophages. Both peripheral blood monocytes and lymphocytes express apoB48R The expression increases during the differentiation from precursor cells to nonocytes, but not during the differentiation from circulating monocytes to macrophages.

Research Products

• [Journal Article] Hyperbaric oxygen induces basic fibroblast growth factor and hepatocyte growth factor expression, and enhances blood perfusion and muscle regeneration in mouse ischemic hind limbs (2007)
  • Author(s): Asano T., Kaneko E., Shinozaki S., Imai Y, Shibayama M., Chiba T., Ai M., Kawakami A., Asaoka H., Nakayama T., Mano Y., Shimokado K.
  • Journal Title: Circ J 71 Pages: 405-411
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Site-specific effect of estradiol on gene expression in the adipose tissue of ob/ob mice (2007)
  • Author(s): Shinozaki S., Chiba T., Kokame K., Miyata T., Kawakami A., Kaneko E., Yoshida M., Shimokado K.
  • Journal Title: Horm Metab Res 39 Pages: 192-196
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A Selective NFkappaB Inhibitor, DHMEQ, Reduced Atherosclerosis in ApoE-deficient mice (2006)
  • Author(s): Chiba T, Kondo Y, Shinozaki S, Kaneko E, Ishigami A, Maruyama N, Umezawa K, Shimokado K.
  • Journal Title: J Atheroscler Thromb. 6 Pages: 308-313
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Favorable effects of early insulin secretion by nateglinide on postprandial hyperlipidemia in patients with type 2 diabetes (2006)
  • Author(s): Ai M, Tanaka A, Ogita K, Shimokado K.
  • Journal Title: Diabetes Care. 5 Pages: 1180
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Metabolic syndrome and the management of traditional risk factors for ischemic heart disease in elderly people (2005)
  • Author(s): Shimokado K.
  • Journal Title: Nippon Ronen Igakkai Zasshi. 42 Pages: 508-11
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Danshen inhibits oxystero1-induced endothelial cell apoptosis in vivo. (2005)
  • Author(s): Nakazawa T, Xui N, Hesong Z, Kinoshita M, Chiba T, Kaneko E, Yui K, Shimokado K.
  • Journal Title: J Atheroscler Thromb. 12 Pages: 132-7
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Insulin signaling in arteries prevents smooth muscle apoptosis. (2005)
  • Author(s): Nakazawa T, Chiba T, Kaneko E, Yui K, Yoshida M, Shimokado K.
  • Journal Title: Arterioscler Thromb Vasc Biol. 25 Pages: 760-5
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Pitavastatin inhibits remnant lipoprotein-induced macrophage foam cell formation through ApoB48 receptor-dependent mechanism. (2005)
  • Author(s): Kawakami A, Tani M, Chiba T, Yui K, Shinozaki S, Nakajima K, Tanaka A, Shimokado K, Yoshida M.
  • Journal Title: Arterioscler Thromb Vasc Biol. 25 Pages: 424-9
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Pitavastatin inhibits remnant lipoprotein-induced macrophage foam cell formation through ApoB48 receptor-dependent mechanism (2005)
  • Author(s): Kawakami A, Tani M, Chiba T, Yui K, Shinozaki S, Nakajima K, Tanaka A, Shimokado K, Yoshida M.
  • Journal Title: Arterioscler Thromb Vasc Biol. 25 Pages: 424-429
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Insulin Signaling in Arteries Prevents Smooth Muscle Apoptosis (2005)
  • Author(s): Nakazawa T, Chiba T, Kaneko E, Yui K, Yoshida M, Shimokado K.
  • Journal Title: Arterioscler Thromb Vasc Biol. 25 Pages: 760-765
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Radical scavengers inhibit oxysterol-induced endothelial apoptosis in vivo (2005)
  • Author(s): Nakazawa, T., Xui Ning, Zeng Hesong, Kinoshita, M., Chiba, T., Kaneko, E, Yui, K., Shimokado, K
  • Journal Title: J Arterioscer Thromb 12 Pages: 132-137
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The expression of apo48 receptor increases the uptake of remnant lipoprotein by cultured hepatocytes (2008)
  • Author(s): Takai M, Shinozaki S, Ishi H, Chiba T, Kawakami A, Yoshida M, Tanaka A, Shimokado K.
  • Organizer: 6^<th> Congress of the Asian Pacific Society of Atherosclerosis and Vascular Diseases
  • Place of Presentation: Hong Kong
  • Year and Date: 2008-09-26
  • Description: 「研究成果報告書概要(欧文)」より