2005 Fiscal Year Final Research Report Summary
T cell epitope of GAD antigen in type 1 diabetes
| Project/Area Number |
16590894
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Keio University |
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Principal Investigator |
SHIMADA Akira Keio University, School of Medicine, Assistant professor, 医学部, 専任講師 (60206167)
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| Co-Investigator(Kenkyū-buntansha) |
KANAZAWA Yasuhiko Keio University, School of Medicine, Assistant professor, 医学部, 助手 (50327508)
OKUBO Yoshiaki Keio University, School of Medicine, Instructor, 医学部, 助手 (20338031)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Type 1 diabetes / GAD / T cell |
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| Research Abstract |
Typical type 1 diabetic patients were recruited after obtaining informed consent and approval of ethics committee of Keio Univ. School of Medicine. Among those patients, 33 year old type 1 diabetic patient with GAD antibody, short duration of disease, and HLA DR9 was selected. Peripheral blood lymphocytes were obtained from the patient, and those were stimulated with recombinant human GAD antigen to establish GAD reactive T cell clones. We could obtain a T cell clone, and confirmed that the phenotype of the clone is CD4, and indeed the clone reacts specifically to GAD antigen. We are now stimulating the clone with GAD peptide to identify the T cell epitopes.
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