2005 Fiscal Year Final Research Report Summary
Detection of feto-maternal immune tolerance by interferon-γ ELISPOT assay
| Project/Area Number |
16590954
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
SHIMAZAKI Chihiro Kyoto Prefectural University of Medicine, Deprtment of Medicine, assistant Professor, 医学研究科, 講師 (50170931)
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| Co-Investigator(Kenkyū-buntansha) |
AKATSUKA Yoshiki Aichi Cancer Center, Department of Tiumor Immunology, Head Reseacher, 腫瘍免疫学部, 主任研究員 (70333391)
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| Project Period (FY) |
2004 – 2005
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| Keywords | feto-maternal immun tolerance / feto-matemal microchimerism / ELISPOT assay / haploidentical transplantation / allogeneic stem cell transplantation |
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| Research Abstract |
Reciprocal cell traffic between mother and fetus during pregnancy gives rise to postpartum fetal-maternal lymphohematopoietic microchimerism, which is frequently detected in blood and tissue from healthy individuals. Recent clinical experiences have suggested the association of microchimerism with acquired immunologic hyporesponsiveness to non-inherited maternal HLA antigens (NIMA) or inherited paternal HLA antigens (IPA). Based on these hypothesis we have started a non-T cell-depleted HLA haploidentical stem cell transplantation between mother and child or between NIMA-mismatched siblings. This study demonstrated a relatively low incidence of severe graft-versus-host disease (GVHD). However, no methods have been established to detect immune hyporesponsiveness to NIMA or IPA in vitro. In the present study, we developed a new method to detect the in vitro T cell reactivity of healthy individuals toward NIMA or IPA using interferon-γ (IFN-γ) Elispot analysis. CD3+ T cell reactivity from healthy individuals is very low toward autologous antigen presenting cells (CD19+ B cells), and is higher toward antigen presenting cells from the third party. CD3+ T cell reactivity toward NIMA is lower than that from the third party, but higher than autologous antigen presenting cells. In addition, CD3+ T cell reactivity toward IPA is similar to that from the third party. These observations suggest that this method using IFN-γ Elispot assay might be useful to speculate the immune tolerance toward NIMA and IPA, and predict GVHD after haploidentical stem cell transplantation between NIMA/IPA-mismatched family members.
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Research Products
(7 results)
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[Journal Article] Non-T-cell-depleted HLA- haploidentical stem cell transplantation after reduced-intensity conditioning in advanced hematological malignancies based on feto-maternal microchimerism.2004
Author(s)
Shimazaki C, Fuchida S, Ochiai N, nakano S, Yamada N, Uchida R, Okamoto M, Okano A, Inaba T, Maruya E, Saji H.
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Journal Title
Br J Haematol 127
Pages: 474-475
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] for the Japan Collaborative Study Group for NIMA-Complementary Haploidentical Stem-Cell Transplantation. Feasibility of HLA-haploidentical hematopoietic stem-cell transplantation between non-inherited maternal antigen (NIMA)- mismatched family members linked with long-term feto-maternal microchimerism.2004
Author(s)
Ichinohe T, Uchiyama T, Shimazaki C, Matsuo K, Tamaki S, Hino M, Watanabe A, Hamaguchi M, Adachi S, Gondo H, Uoshima N, Yoshihara T, Hatanaka K, Fujii H, Kawa K, Kawanishi K, Oka K, Kimura H, Itoh M, Inukai T, Maruya E, Saji H, Kodera Y
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Journal Title
Blood 104
Pages: 3821-3828
Description
「研究成果報告書概要(欧文)」より
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