• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

The mechanism of induction of ITP by H.pylori infection.

Research Project

Project/Area Number 16590989
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionOita University

Principal Investigator

OGATA Masao  Oita University, Faculty of medicine, Research assistant, 医学部, 助手 (10332892)

Co-Investigator(Kenkyū-buntansha) SATO Ryugo  Oita University, Faculty of medicine, Research assistant, 医学部, 助手 (00325705)
MURAKAMI Kazunari  Oita University, Faculty of medicine, Assistant Professor, 医学部, 助教授 (00239485)
GOTOH Kazuyo  Oita University, Institute of Scientific Research, Research assistant, 総合科学研究支援センター, 助手 (30381031)
Project Period (FY) 2004 – 2005
KeywordsITP / H.pylori / EBV / BLC / TNF-α
Research Abstract

To investigate the possibility of an interaction between two ubiquitous human pathogens, Helicobacter pylori (H.pylori) and Epstein-Barr virus (EBV), the effect of monochloramine, locally produced by H.pylori infection, on gastric epithelium latently infected with EBV was examined, by assessing the induction of EBV lytic infection. AGS cells harbouring latently infected EBV were used as the indicator of lytic change caused by monochloramine treatment. Lytic infection, determined by morphological change and EA-D antigen expression, occurred immediately after treatment with in vitro-synthesized monochloramine. Analysis of EBV infection in human gastric tissue revealed that out of 48 H.-pylori-positive patients, 24 were positive for EBER-1, and 18 and 13 were positive for EBNA1 and LMP-a antigen, respectively. The results suggest that H.pylori-associated monochloramine induces EBV lytic conversion in gastric epithelium latently infected with EBV.
Helicobacter pylori is a gram-negative bacterium that colonizes the human stomach and causes chronic gastric diseases. It is recognized as the etiologic agent of atrophic gastritis, peptic ulcer, intestinal metaplasia, MALToma and gastric cancer. Various pathogenic proteins of H.pylori have been reported, such as VacA, CagA, LPS, HSP60 and unease. Dendritic cells (DCs) are potent antigen-presenting cells that initiate acquired immune responses. Previously HP-MP1 has been reported as a new proinflammatory mediator in stomach infected with H.pylori and HP0596 gene is homologous to HP-MP1. We designated the HP0596 gene as the TNF-a inducing protein (Tipa) and Tipa was present in the culture medium of H.pylori. We also reported that Monomer Tipa is 19kDa-sized protein located in H.pylori membrane fraction and forms homodimer structure due to two cysteine residues in N-terminal site. In this study, we report here an immunomodulatory effect of novel Tipa protein of H.pylori to DCs.

  • Research Products

    (10 results)

All 2006 2005 2004

All Journal Article (10 results)

  • [Journal Article] Helicobacter pylori-associated oxidant monochloramine induces reactivation of Epstein Barr virus in gastric epithelial cells latently infected with EBV2006

    • Author(s)
      Junko minoura-Eto, Kazuyo Gotoh, Ryugo Sato, Masao Ogata, Toshio Fujioka, Akira Nishizono
    • Journal Title

      The Journal of Medical Microbiology (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Human Herpesvirus 6 DNA in Plasma after Allogeneic Stem Cell Transplantation : Incidence and Clinical Significance2006

    • Author(s)
      Masao Ogata, Hiroshi Kikuchi, Takako Satou, Rie Kawano, Junji Ikewaki, Kazuhiro Kohno, Kenji Kashima, Eiichi Ohtsuka, Jun-ichi Kadota
    • Journal Title

      The Journal of Infectious Diseases 193

      Pages: 68-79

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ^*Helicobacter pylori-associated oxidant monochloramine induces reactivation of Epstein Barr virus in gastric epithelial cells latently infected with EBV2006

    • Author(s)
      Junko minoura-Eto, Kazuyo Gotoh, Ryugo Sato, Masao Ogata, Toshio Fujioka, Akira Nishizono.
    • Journal Title

      The Journal of Medical Microbiology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ^* Human Herpesvirus 6 DNA in Plasma after Allogeneic Stem Cell Transplantation : Incidence and Clinical Significance2006

    • Author(s)
      Masao Ogata, Hiroshi Kikuchi, Takako Satou, Ric Kawano, Junji Ikewaki, Kazuhiro Kohno, Kenji Kashima, Eiichi Ohtsuka, Jun-ichi Kadota.
    • Journal Title

      The Journal of Infectious Diseases 193

      Pages: 68-79

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Real-time PCR assays based on distinct genomic regions for cytomegalovirus reactivation following hematopoietic stem cell transplantation.2005

    • Author(s)
      Ikewaki J, Ohtsuka E, Satou T, Kawano R, Ogata M, Kikuchi H, Nasu M.
    • Journal Title

      Bone Marrow Transplant Feb.35(4)

      Pages: 403-410

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ^* Real-time PCR assays based on distinct genomic regions for cytomegalovirus reactivation following hematopoietic stem cell transplantation.2005

    • Author(s)
      Ikewaki J, Ohtsuka E, Satou T, Kawano R, Ogata M, Kikuchi H, Nasu M.
    • Journal Title

      Bone Marrow Transplant Feb.35(4)

      Pages: 403-410

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Response to Cyclosporine Therapy in Patients with Myelodysplastic Syndrome : A Clinical Study of 12 Cases and Literature Review2004

    • Author(s)
      Masao Ogata, Eiichi Ohtsuka, Tomoyuki Imamura, Junji Ikewaki, Yuko Ogata, Kazuhiro Kohno, Toshiyuki Nakayama, Keiji Ono, Yoshio Saburi, Hiroshi Kikuchi, Masaru Nasu
    • Journal Title

      International Journal of Hematology 80(1)

      Pages: 35-42

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of Helicobacter pylori Eradication on Platelet Recovery in Patients With Chronic Idiopathic Thrombocytopenic Purpura.2004

    • Author(s)
      Sato R, Murakami K, Watanabe K, Okimoto T, Miyajima H, Ogata M, Ohtsuka E, Kodama M, Saburi Y, Fujioka T, Nasu M.
    • Journal Title

      Arch Intern Med Sept.27 174(17)

      Pages: 1904-1907

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ^* Response to Cyclosporine Therapy in Patients with Myelodysplastic Syndrome : A Clinical Study of 12 Cases and Literature Review2004

    • Author(s)
      Masao Ogata, Eiichi Ohtsuka, Tomoyuki Imamura, Junji Ikewaki, Yuko Ogata, Kazuhiro Kohno, Toshiyuki Nakayama, Keiji Ono, Yoshio Saburi, Hiroshi Kikuchi, Masaru Nasu.
    • Journal Title

      International Journal of Hematology 80(1)

      Pages: 35-42

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ^* Effect of Helicobacter pylori Eradication on Platelet Recovery in patients With Chronic Idiopathic Thrombocytopenic Purpura.2004

    • Author(s)
      Sato R, Murakami K, Watanabe K, Okimoto T, Miyajima H, Ogata M, Ohtsuka E, Kodama M, Saburi Y, Fujioka T, Nasu M.
    • Journal Title

      Arch Intern Med Sept.27, 174(17)

      Pages: 1904-1907

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi