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2006 Fiscal Year Final Research Report Summary

Research for the medical treatment of organic fibrosis by controlling the protein interacting with connective tissue growth factor

Research Project

Project/Area Number 16591000
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionKinki University

Principal Investigator

YOSHIDA Koji  Kinki University, medicine, lecturer, 医学部, 講師 (60230736)

Co-Investigator(Kenkyū-buntansha) SAITO Akio  Kinki University, medicine, lecturer, 医学部, 講師 (40153788)
HAYAKAWA Sumio  Kinki University, medicine, assistant, 医学部, 助手 (00368292)
NAKATANI Tatsuya  Kinki University, medicine, lecturer, 医学部, 講師 (40319661)
Project Period (FY) 2004 – 2006
KeywordsCTGF / fibronectin / fibrin / two-hybrid
Research Abstract

Connective tissue growth factor (CTGF) is a member of the CCN family of the cysteine-rich proteins and involved in wound healing and fibrosis. We have previously shown a biochemical interaction between the CTGF and fibronectin (FN) using the yeast two-hybrid system. In this study, we confirmed the interaction between the CTGF and FN using the surface plasmon resonance (SPR) and solid-phase binding analysis. Our results show that the regions containing the FN type I repeat modules (the N-terminal fibrin, the gelatin-collagen and the C-terminal fibrin binding domains) of FN and the C-terminal domain of CTGF are required for the interaction. We also demonstrated that CTGF enhances the affinity of FN to fibrin. It appears that CTGF contributes to the extracellular matrix accumulation in wound healing and tissue fibrosis by enhancing the affinity of FN to fibrin. Because CTGF is up-regulated during the tissue repair and in coagulation cascade-associated fibrotic disorders, the new function of CTGF found in this study is consistent with its physiological role."

  • Research Products

    (2 results)

All Other

All Journal Article (2 results)

  • [Journal Article] Connective tissue growth factor binds to fibronectin through the type I repeat. modules and enhances the affinity of fibronectin to fibrin

    • Author(s)
      Koji Yoshida, Hiroshi Munakata
    • Journal Title

      Biochim. Biophys. Acta (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Connective tissue growth factor binds to fibronectin through the type I repeat modules and enhances the affinity of fibronectin to fibrin

    • Author(s)
      Koji Yoshida, Hiroshi Munakata
    • Journal Title

      Biochim.Biophys.Acta (in press)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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