2005 Fiscal Year Final Research Report Summary
Analysis of the protein associated with Kawasaki disease and the coronary lesion using ProteinChip technology.
| Project/Area Number |
16591061
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Nippon Medical School |
|---|
Principal Investigator |
KAMISAGO Mitsuhiro Nippon Medical School, Department of Medicine, Assistant professor, 医学部, 講師 (20256928)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ASANO Takeshi Nippon Medical School, Department of Medicine, Associate Professor, 医学部, 助教授 (70277490)
OGAWA Shunichi Nippon Medical School, Department of Medicine, Professor, 医学部, 教授 (50194436)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Keywords | Kawasaki disease / ProteinChip / protein expression analysis |
|---|
| Research Abstract |
To identify the protein associated with Kawasaki disease, we performed Expression Difference Mapping analysis on serum samples from patients using ProteinChip technology. Methods : Pre-treatment serum samples were obtained from 14 children with Kawasaki disease (before intravenous immunoglobulin therapy), 8 of them (after IVIG therapy), 6 children of other inflammatory illnesses with elevated CRP, 2 of Henoch-Schonlain disease. Strong anion-exchange (Q10), weak cation-exchange (CH10) and immobilized affinity capture (IMAC30) ProteinChip Arrays were used, respectively. We also tried many conditions of Binding/Washing Buffer to get significant results. Sinapinic acid was added to each spot of these arrays as energy absorbing molecule. SELDI (Surface Enhanced Laser Desorption/Ionization)-TOF-MS (Time of Flight Mass Spectrometer) was used to create protein profiles of each samples. To analyze protein expression of Kawasaki disease, we compared the result from acute phase with that from convalescent phase. Results : In the protein expression profiling of Kawasaki disease, two peaks (11.5kD, 11.6kD) were higher in acute phase than in convalescent phase. One peak (23.5kD) was lower in acute phase. However comparing the profiling of Kawasaki disease (acute phase) to that of other inflammatory illness with elevated CRP, the former differences were not detected. On the other hand, the peak of 23.5kD was also lower in acute phase of Kawasaki disease. To purify and identify these proteins and verify these results further study will be needed.
|
