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2005 Fiscal Year Final Research Report Summary

Analysis of pathomechanism of androgenetic alopecia by investigating TGF-β1 promoter

Research Project

Project/Area Number 16591098
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionOsaka University

Principal Investigator

INUI Shigeki  Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (30324750)

Co-Investigator(Kenkyū-buntansha) ITAMI Satoshi  Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (30136791)
Project Period (FY) 2004 – 2005
Keywordsandrogen receptor / androgenetic alopecia / TGF-β1 promoter / dermal papilla cell / reporter plasmid / AP-1
Research Abstract

We reported that TGF-β1 is up-regulated by androgen in dermal papilla cells (DPCs) from androgenetic alopecia (AGA) using our coculture system of DPCs transiently transfected with androgen receptor (AR) expression vector and keratinocytes (KCs). This finding suggested that the regulation of TGF-β1 by androgen is a key step in the pathogenesis of AGA. To examine the possible regulatory mechanism of TGF-β1 gene expression by androgen, we analysed TGF-β1 promoter activity of the pTGF-β1-Luc vector, which was constructed by connecting the 5' upstream region of the TGF-β1 gene (-1362 bp upstream from the transcription start site) to the luciferase reporter gene. When pTGF-β1-Luc and AR expression vector were cotransfected in DPCs from AGA, R1881 increased luciferase activity to around 10-fold. In contrast, this induction did not occur in CV-1 cells and transformed DPCs. Thus the regulation of promoter activity by androgen may be cell-specific. From analysis of deleted pTGF-β1-Luc vector using DPCs from AGA, there are two possible regulatory regions (-1131〜-731 and -459〜 -323) and two negative regulatory regions (-1326〜-1127 and -735〜-459). We found two AP-1 binding sites in the region at -459〜-323. Using the reporter vector containing the mutation at these two AP-1 sites, we found that these sites are involved in the suppression of TGF-β1 promoter and that AR can release this suppression.

  • Research Products

    (10 results)

All 2006 2005

All Journal Article (6 results) Book (4 results)

  • [Journal Article] Role of androgen in mesenchymal epithelial interactions in human hair follicle2005

    • Author(s)
      Itami S
    • Journal Title

      J Investig Dermatol Symp Proc 10.3

      Pages: 209-211

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] LRIG-1 provides a novel prognostic predictor in squamous cell carcinoma of the skin : an immunohistochemical analysis for 38 cases2005

    • Author(s)
      Tanemura A
    • Journal Title

      Dermatol Surg 31.4

      Pages: 423-430

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ruta graveolens L.の育毛効果2005

    • Author(s)
      多葉田誉
    • Journal Title

      FRAGRANCE JOURNAL 33.12

      Pages: 58-62

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Role of androgen in mesenchymal epithelial interactions in human hair follicle2005

    • Author(s)
      Itami S et al.
    • Journal Title

      J Investig Dermatol Symp Proc 10

      Pages: 209-211

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] LRIG-1 provides a novel prognostic predictor in squamous cell carcinoma of the skin : an immunohistochemical analysis for 38 cases2005

    • Author(s)
      Tanemura A et al.
    • Journal Title

      Dermatol Surg 31

      Pages: 423-430

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hair Growth Stimulation by Ruta graveolens L.2005

    • Author(s)
      Tabata H et al.
    • Journal Title

      Fragrance Journal 33

      Pages: 58-62

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 脱毛症のすべて2006

    • Author(s)
      乾 重樹
    • Total Pages
      17-21
    • Publisher
      全日本病院出版会
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 毛の悩みに応える皮膚科診療 毛髪最前線2006

    • Author(s)
      板見 智
    • Publisher
      男性はなぜ禿げる?-男性型脱毛の病態(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Alopecia2006

    • Author(s)
      Inui S
    • Total Pages
      17-21
    • Publisher
      MB Derma
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Why do males get hair loss? : Pathomecanism of androgenetic alopecia2006

    • Author(s)
      Itami S et al.
    • Publisher
      Hair bilogy medicine (in press)
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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