2006 Fiscal Year Final Research Report Summary
Development of specific, universal targeted radiotherapy through induction of avidin on cancer cell membrane and internalization of scavenger receptor
| Project/Area Number |
16591194
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kanazawa University |
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Principal Investigator |
KINUYA Seigo Kanazawa University, Graduate School of Medical Science, Professor, 医学系研究科, 教授 (20281024)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Keiichi Kanazawa University, Graduate School of Medical Science, Professor, 医学系研究科, 教授 (30204663)
WASHIYAMA Koshin Kanazawa University, Graduate School of Medical Science, Assisstant Professor, 医学系研究科, 助手 (80313675)
YOSHIMOTO Mitsuyoshi Kanazawa University, Graduate School of Medical Science, Assisstant Professor, 医学系研究科, 助手 (00345638)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Cancer / Radiopharmaceuticals / Avidin / Targered radiotherapy / Scavidin / Scavenger / スカベンジャー |
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| Research Abstract |
1. We aimed to develop effective non-toxic targeted radiotherapy for malignant tumors. A complex of avidin that has intense binding affinity to biotin and scavenger receptors that would internalize radiolabeled biotin into cells via endocytosis processs. In order to attain this complex named Scavidin, genes determining each molecules were cloned. That is hSR-A-II for human scavenger receptor. Total RNA fraction was extracted from human monocytic leukemia cells, THP-1, after loading 12-o-tetradecanoyl phorbol-13-acetate. hSR-A-II cDNA was amplified by PCR. Avidin cDNA was similarly cloned from materials of fallopian tubes of chicken. A complex coding Scavidin was then obtained by inserting avidin cDNA so that endocytotic functions of hSR-A-II would not be destroyed.
2. Characteristics of radionuclides labeled to targeting molecules extremely affect both effectiveness and toxicity of targeted radiotherapy. Superiority of targeted radiotherapy with ^<186>Re to that with ^<131>I was determined in animal models bearing colon cancer metastases.
3. Pre-therapeutic prediction of therapeutic outcomes is a key for so-called tailor-made management of cancer patients ; therefore, scintigraphic assessment with a cationic compound, "mTc-sestamibi, was investigated.
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