The analysis of TLR and TCR Vβ repertoire which would determinate and innate immunity

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 16591277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: ARUGA Atsushi Tokyo Women's Medical University, Graduate School of Medical Science, Professor (40221056)
• Project Period (FY): 2004 – 2006
• Keywords: Toll like receptor / TCR Vβ / innate immunity / γδ T cell / dendritic cell

Research Abstract

An innate immunity against bacteria, virus and abnormal cells is carried on the macrophage, dendritic cell, neutrophil, NK cell and γδT cell which belong to white blood cell. They have the receptor against each antigen named toll-like receptor (TLR). In human system, the expressions of TLR1~9 are not equal and that make the differences of cytokine release from the cells when their TLR meet the ligand. These differences of TLR expression would decide the clinical progress in the patients with infection or cancer. As well as the cytokine release, the signal transduction from TLR would activate MyD88 and NF-kB gene, and induce the expression of cell surface molecules which could activate the NK cells or γδT cells.
The analysis of TCR Vβ repertoire was also done in the same patients. There were differences in the populations of each TCR Vβ. However, any correlation between TLR expression and TCR Vβ repertoire were not seen in this study. Especially, it was suggested that TCR Vβ repertoire would make an important role to induce a required immunity.
From now on, the analysis of TLR expression and TCR Vβ repertoire in the patients of cancer or infection will be needed to assess their immunological function to develop a new strategy for cancer and infection.