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2005 Fiscal Year Final Research Report Summary

Roles of P27/Skp2 pathway and identification of target genes on tumor malignancy of colon cancer

Research Project

Project/Area Number 16591308
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

KAMIYA Kinji  Hamamatsu University School of Medicine, Medicine, Instructor, 医学部, 助手 (20324361)

Co-Investigator(Kenkyū-buntansha) KONNO Hiroyuki  Hamamatsu University School of Medicine, Medicine, Professor, 医学部, 教授 (00138033)
KITAGAWA Kyoko  Hamamatsu University School of Medicine, Medicine, Instructor, 医学部, 助手 (20299605)
KITAGAWA Masatoshi  Hamamatsu University School of Medicine, Medicine, Professor, 医学部, 教授 (50294971)
Project Period (FY) 2004 – 2005
Keywordscolon cancer / gastric cancer / Skp2 / p27
Research Abstract

Ubiquitin-proteasome pathway is a key system that controls cellular levels of number of proteins. This pathway is responsible not only for the degradation of short-lived proteins but also tumor suppressors, transcription factors and cell cycle proteins. Recently, it has been reported that degradation of Cdk inhibitory protein p27^<Kip1> is correlated with the prognosis of colon cancer. In this study, we investigated the molecular mechanisms of down-regulation of p27^<Kip1> and acquirement of tumor malignancy in the colon cancer.
We established two cell lines, p27-hKO,p27 hetero-knockout HCT116 human colon cancer cell line and HCT-Skp2, transfected with Skp2 expression vector into HCT116 cells. We found the degradation of p27 protein in p27-hKO,HCT-Skp2 cells. Also we found that the invasive ability of p27-hKO,HCT-Skp2 cells is higher than HCT116 cells. Furthermore we applied comprehensive gene expression analysis between p27-hKO and HCT116 using microarray. As a result, function-unknown genes were overexpressed, and we demonstrated that the invasion ability of cancer cells was further increased by transfection of these genes.

  • Research Products

    (20 results)

All 2006 2005

All Journal Article (20 results)

  • [Journal Article] Effect of MdmX on Mdm2-mediated downregulation of pRB2006

    • Author(s)
      Uchida, C. et al.
    • Journal Title

      FEBS Letters 560

      Pages: 1753-1758

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ubiquitin-dependent degradation of adenovirus E1A protein is inhibited by BS692006

    • Author(s)
      Isobe, T. et al.
    • Journal Title

      Biochem Biophys Res Commun 339

      Pages: 367-374

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mdm2-mediated pRB downregulation is involved in carcinogenesis in a p53-independent manner2006

    • Author(s)
      Miwa, S. et al.
    • Journal Title

      Biochem Biophys Res Commun 340

      Pages: 54-61

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunohistochemical and geneticfeatures of gastric and the metastatic liver GISTs, sequential analyses2006

    • Author(s)
      Kikuchi, H. et al.
    • Journal Title

      Cancer Sci 97

      Pages: 127-132

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Automated bedside measurement of penile blood flow using pulse-volume plethysmography2006

    • Author(s)
      Unno N.et al.
    • Journal Title

      Surg Today 36

      Pages: 257-261

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intestinal Behcet's disease with pyoderma gangrenosum : a case report2006

    • Author(s)
      Nakamura T. et al.
    • Journal Title

      World J Gastroenterol 12

      Pages: 979-981

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Left paraduodenal hernia in an adult complicated by ascending colon cancer : A case report2006

    • Author(s)
      Kurachi K. et al.
    • Journal Title

      World J Gastroenterol 12

      Pages: 1795-1797

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intraoperative monitoring of penile and buttock blood flow during endovascular abdominal aortic aneurysm repair2006

    • Author(s)
      Inuzuka K. et al.
    • Journal Title

      Eur J Vasc Endovasc Surg 31

      Pages: 359-365

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ubiquitin-dependent degradation of adenovirus E1A protein is inhibited by BS692006

    • Author(s)
      Isobe, T. et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 339

      Pages: 367-374

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mdm2-mediated pRB downregulation is involved in carcinogenesis in a p53-independent manner2006

    • Author(s)
      Miwa, S. et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 340

      Pages: 54-61

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunohistochemical and geneticfeatures of gastric and the metastatic liver GISTs, sequential analyses2006

    • Author(s)
      Kikuchi, H. et al.
    • Journal Title

      Cancer Sci. 97

      Pages: 127-132

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Automated bedside measurement of penile blood flow using pulse-volume plethysmography2006

    • Author(s)
      Unno N.et al.
    • Journal Title

      Surg.Today 36

      Pages: 257-261

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intestinal Behcet's disease with pyoderma gangrenosum : a case report2006

    • Author(s)
      Nakamura T. et al.
    • Journal Title

      World J.Gastroenterol. 12

      Pages: 979-981

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Left paraduodenal hernia in an adult complicated by ascending colon cancer : A case report2006

    • Author(s)
      Kurachi K. et al.
    • Journal Title

      World J.Gastroenterol. 12

      Pages: 1795-1797

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intraoperative monitoring of penile and buttock blood flow during endovascular abdominal aortic aneurysm repair2006

    • Author(s)
      Inuzuka K. et al.
    • Journal Title

      Eur.J.Vasc.Endovasc.Surg. 31

      Pages: 359-365

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Peritoneal lavage with oxygenated perfluorochemical improves hemodynamics, intestinal injury, and survival in a rat model of severe hemorrhagic shock and resuscitation2005

    • Author(s)
      Yamamoto N. et al.
    • Journal Title

      Shock 24

      Pages: 171-176

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transcriptional induction of Smurf2 ubiquitin ligase by TGF-β2005

    • Author(s)
      Ohashi, N. et al.
    • Journal Title

      FEBS Letters 579

      Pages: 2557-2563

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Association of interleukin-8 and plasminogen activator system in the progression of colorectal cancer2005

    • Author(s)
      Terada H. et al.
    • Journal Title

      Eur Surg Res 37

      Pages: 166-172

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The FWD1/β-TrCP-mediated degradation pathway establishes a turning off switch of a Cdc42 guanine nucleotide exchange factor, FGD12005

    • Author(s)
      Hayakawa, M. et al.
    • Journal Title

      Genes to Cells 10

      Pages: 241-251

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Association of interleukin-8 and plasminogen activator system in the progression of colorectal cancer2005

    • Author(s)
      Terada H. et al.
    • Journal Title

      Eur.Surg.Res. 7

      Pages: 166-172

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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