2006 Fiscal Year Final Research Report Summary
The study of molecular marker expression in evaluation for the malignancy potential of colorectal cancer
| Project/Area Number |
16591357
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
SAITO Noboru Tokyo Women's Medical University, School of Medicine, Lecturer, 医学部, 講師 (10225724)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Nami Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助手 (80307553)
HIROSAWA Tomoichiro Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助手 (50307550)
YAMADA Akiteru Tokyo Women's Medical University, School of Medicine, Assistant Proefssor, 医学部, 助手 (30408562)
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| Project Period (FY) |
2004 – 2006
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| Keywords | colorectal cancer / adhesion molecule / cell growth factor / molecular marker / laminin / TGF-β / HGF / EGF |
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| Research Abstract |
We investigated the molecular marker expression in evaluation for the maliginancy potential of colorectal cancer.
1. HGF(hepatocyte growth factor), EGF(epidermal growth factor)
(1) Serological analysis demonstrated that preoperative serum HGF and EGF level was significantly higher in the cancer group than in the control group (p < 0.0001).
(2) Immunohistochemical analysis demonstrated the strong expression and colocalization of HGF, c-Met and HGF in the cancer cells.
2. TGF-β
(1) In immunohistological staining, the positive rate became higher in lymph-node metastases and in progressing invasion into the vascular system.
(2) In serological examination, a significant difference was found between cancer cases and non-cancer cases, and TGF-β1 level rose with progressing liver metastasis and venous invasion.
3. Laminin
(1) Mean serum laminin in subjects with colon cancer was 606.3±260.2 ng/ml, significantly higher than that of 258.0±92.0 ng/ml in normal controls (p<0.0001).
(2) The positive rate was higher at 89.3% for laminin vs 38.0% for CEA and 19.5% for CA19-9.
(3) Mean serum laminin in subjects with hepatic metastases was 668.0±274.7 ng/ml, significantly higher than that of 585.2±252.5 ng/ml in subjects without hepatic metastases (p=0.0472).
(4) Survival rates were significantly lower in the high (【greater than or equal】520 ng/ml) than in the low laminin group (<350 ng/ml) (P=0.0451). Univariate and multivariate analysis, using Cox' s proportional hazard regression model, showed serum laminin is an independent prognostic factor in colorectal cancer, along with hepatic, pulmonary and peritoneal metastases.
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