2005 Fiscal Year Final Research Report Summary
Research of the sentinel lymph node biopsy in the gastrointestinal cancer, breast cancer and thyroid cancer : Analysis using real-time RT-PCR
| Project/Area Number |
16591370
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Aichi Medical University |
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Principal Investigator |
NAKANO Shogo Aichi Medical University, Department of breast and endocrine surgery, assistant professor, 医学部, 助教授 (20351108)
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| Project Period (FY) |
2004 – 2005
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| Keywords | follicular adenoma / follicular carcinoma / CEA mRNA / RT-PCR |
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| Research Abstract |
BACKGROUND : We prospectively tested whether circulating tumor cells could be detected in peripheral blood of patients with thyroid tumors by a reverse transcription-polymerase chain reaction (RT-PCR) to detect carcinoembryonic antigen (CEA) messenger RNA (mRNA). METHODS : We assayed for CEA mRNA by RT-PCR in peripheral blood sampled before and 2 to 3 weeks after curative surgery for thyroid tumors in 121 patients. Blood samples from 7 patients with chronic thyroiditis and 7 healthy subjects served as controls. RESULTS : No control samples were positive for CEA mRNA by RT-PCR. Of 121 preoperative samples from patients with thyroid tumor, 6 were positive (5.0%). Preoperative frequencies of CEA mRNA positivity in benign tumor, papillary carcinoma, follicular variant papillary carcinoma, minimally invasive follicular carcinoma, and widely invasive follicular carcinoma were 0%, 0%, 0%, 44.4% (4/9), and 50.0% (2/4), respectively. Among positive patients only one, who had widely invasive follicular carcinoma, remained positive after surgery. CONCLUSIONS : RT-PCR detection of tumor cells in preoperative blood often can distinguish malignant from benign follicular thyroid tumors.
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