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2005 Fiscal Year Final Research Report Summary

Enhancement of ACL graft healing with synovium-derived mesenchymal stem cells

Research Project

Project/Area Number 16591477
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTokyo Medical and Dental University

Principal Investigator

MUNETA Takeshi  Tokyo Med & Dent Univ, Orthop Sung, Professor, 大学院・医歯学総合研究科, 教授 (50190864)

Co-Investigator(Kenkyū-buntansha) SEKIYA Ichiro  Tokyo Med & Dent Univ, Orthop Surg, Assistant Prof, 大学院・医歯学総合研究科, 助手 (10345291)
TAKAKUDA Kazuo  Tokyo Med & Dent Univ, Institute of Biomat & Bioengineering, 生体材料工学研究所, 教授 (70108223)
Project Period (FY) 2004 – 2005
KeywordsMesenchymal stem cells / healing enhancement / tendon transfer / ACL injury / ACL reconstruction / histological analysis / cell therapy
Research Abstract

Purpose : To investigate the effect and mechanism of the implantation of the synovium-derived mesenchymal stem cells (MSCs) on tendon-bone healing in rats. Type of Study : Experimental study. Methods : Achilles' tendon grafts of 19 Sprague-Dawley rats were inserted into a bone tunnel from the tibial plateau to the tibial tuberosity with a pull-out fixation. The bone tunnel was filled with MSCs labeled with Dil or without MSCs as the control. Histological analyses (n=3) by microscopy and fluorescent microscopy were-performed. Results : Histological analyses showed MSCs improved tendon-bone healing by increasing the production of collagen fibers at 1 week and forming more perpendicular collagen fibers connecting the bone to tendon resembling Sharpey's fibers at 2 weeks. Implanted MSCs were observed in the tendon-bone interface at 1 and 2 weeks. Labeled cells were decreased and the effect of implanted cells disappeared at 4 weeks. Conclusions : Implanting synoviam-derived MSCs on tendon-bone interface resulted in an increased bone-tendon healing in the early phase by producing more collagen fibers. Clinical relevance : Enhancement of bone-tendon healing could provide accelerated rehabilitation after anterior cruciate ligament (ACL) reconstruction using hamstring tendon.

  • Research Products

    (10 results)

All 2006 2005 2004

All Journal Article (10 results)

  • [Journal Article] In vitro chondrogenesis of human synovium-derived mesenchymal stem cells : Optimal condition and comparison with bone marrow-derived cells.2006

    • Author(s)
      Shirasawa Shinichi
    • Journal Title

      Journal of Cellular Biochemistry 97(1)

      Pages: 84-97

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Higher chondrogenic potential of fibrous synovium- and adipose synovium-derived cells compared with subcutaneous fat-derived cells : distinguishing properties of mesenchymal stem cells in humans.2006

    • Author(s)
      Mochizuki Tomoyuki
    • Journal Title

      Arthritis and Rheumatism 54(3)

      Pages: 843-53

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In vitro chondrogenesis of human synovium-derived mesenchymal stem cells : optimal condition and comparison with bone marrow-derived cells.2006

    • Author(s)
      Shirasawa S, et al.
    • Journal Title

      Journal of Cellular Biochemistry 97

      Pages: 84-97

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Higher chondrogenic potential of fibrous synovium and adipose synovium-derived cells compared with subcutaneous fat-derived cells : distinguishing properties of mesenchymal stem cells in human2006

    • Author(s)
      Mochizuki Tomoyuki, et al.
    • Journal Title

      Arthritis and Rheumatism 54

      Pages: 843-853

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Comparison of human stem cells derived from various mesenchymal tissues : superiority of synovium as a cell source2005

    • Author(s)
      Sakaguchi Yusuke
    • Journal Title

      Arthritis and Rheumatism 52(8)

      Pages: 2521-2529

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In vitro cartilage formation of composites of synovium-derived mesenchymal stem cells with collagen gel2005

    • Author(s)
      Yokoyama Akiko
    • Journal Title

      Cell and Tissue Research 322(2)

      Pages: 289-98

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Comparison of human stem cells derived from various mesenchymal tissues : superiority of synovium as a cell source2005

    • Author(s)
      Sakaguchi Y, et al.
    • Journal Title

      Arthritis and Rheumatism 52

      Pages: 2521-2529

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vitro cartilage formation of composites of synovium-derived mesenchymal stem cells with collagen gel2005

    • Author(s)
      Yokoyama A, et al.
    • Journal Title

      Cell and Tissue Research 322

      Pages: 289-298

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Suspended cells from trabecular bone by collagenase digestion become virtually identical to mesenchymal stem cells obtained from marrow aspirates2004

    • Author(s)
      Sakaguchi Yusuke
    • Journal Title

      Blood 104(9)

      Pages: 2728-2735

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Suspended cells from trabecular bone by collagenase digestion became virtually identical to mesenchymal stem cells obtained from marrow aspiration2004

    • Author(s)
      Sakaguchi Y, et al.
    • Journal Title

      Blood 104

      Pages: 2728-2735

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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