2007 Fiscal Year Final Research Report Summary
Osteochondral regeneration using autologous cell construct with β TCP
| Project/Area Number |
16591497
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
MATSUDA Shuichi Kyushu University, Department of Orthopaedic Surgery, Assistant Professor (40294938)
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| Project Period (FY) |
2004 – 2007
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| Keywords | cartilage repair / mesenchymal stem cell / TCP / type II collagen / chondrocyte |
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| Research Abstract |
We established a new method to make cell only construct on TCP to repair osteochondral defect.
Mesenchymal stem cells (MSCs) were obtained from the iliac crest of Japanese-white-rabbit. Monolayer expanded cells were mold into a cylinder of 4.8mm in diameter and 4mm in height on TCP. The autologous labeled-MSC plug was implanted with TCP in the patellofemoral groove of the rabbit. The knees were immobilized for one week. The rabbits were sacrificed at 3, 6, 12, 24, and 52 weeks after operation. The specimens were evaluated histologically and scanned with micro CT.
In gross appearance of the cartilage at 6 weeks, most of the surface of the defect was covered with cartilage-like tissue. Smooth surfaces were maintained in regenerated cartilage at one year. Histologically, columnar distribution of the chondrocyte was observed in regenerated hyaline cartilage. In the subchondral bone level, endochondral ossification occurred at the peripheral area of the transplants, and small area in the central remained hyaline cartilage. Micro CT showed further ossification in subchondral bone level. Confocal microscopy detected the labeled cells both in cartilage layer and subchondral bone layer.
The current study suggests that this cell-delivery system can be used for treatment of osteochondral defect and possibly for osteoarthritis.
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Research Products
(60 results)
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[Journal Article] Assessment of Anterolateral Rotatory Instability in the Anterior Cruciate Ligament-Deficient Knee Using an Open Magnetic Resonance Imaging System2007
Author(s)
Okazaki, K., Miura, H., Matsuda, S., Yasunaga, T., Nakashima, H., Konishi, K., Iwamoto, Y., Hashizume, M
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Journal Title
Am J Sports Med 35(7)
Pages: 1091-7
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Asymmetry of mediolateral laxity of the normal knee2006
Author(s)
Okazaki, K., Miura, H., Matsuda, S., Takeuchi, N., Mawatari, T., Hashizume, M., Iwamoto, Y
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Journal Title
J Orthop Sci 11(3)
Pages: 264-266
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Anteroposterior stability in posterior cruciate ligament-retaining total knee arthroplasty2006
Author(s)
Mizu-uchi, H., Matsuda, S., Miura, H., Nabeyama, R., Okazaki, K., Iwamoto, Y
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Journal Title
J Arthroplasty 21
Pages: 592-598
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The extramedullary guide of the proximal tibia resection should be seen in front during total knee arthroplasty2006
Author(s)
Nagamine, R., Matsunobu, T., Takayama, M., Miura, H., Matsuda, S., Iwamoto, Y
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Journal Title
Fukuoka Acta Medica 97
Pages: 146-151
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional evaluation of four patients treated with V-Y quadricepsplasty in total knee arthroplasty2006
Author(s)
Tsukamoto, N., Miura, H., Matsuda, S., Mawatari, T., Kato, H., Iwamoto, Y
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Journal Title
J Orthop Sci. 11(4)
Pages: 394-400
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The Effect of Ankle Rotation on Cutting Error of the Tibia in Total Knee Arthroplasty2006
Author(s)
Mizu-uchi, H., Matsuda, S., Miura, H., Higaki, H., Okazaki, K., Iwamoto, Y
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Journal Title
J Bone and Joint Surg Am 88
Pages: 2632-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Importance of the lateral anatomic tibial slope as a guide to the tibial cut in total knee arthroplasty in Japanese patients2005
Author(s)
Kuwano, T., Urabe, K., Miura, H., Nagamine, R., Matsuda, S., Satomura, M., Sasaki, T., Sakai, S., Honda, H., wamoto, Y
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Journal Title
J Orthop Sci. 10(1)
Pages: 42-7
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Contact stress at the post-cam mechanism in posterior stabilized total knee arthroplasty2005
Author(s)
Nakayama, K., Matsuda, S., Miura, H., Higaki, H., Otsuka, K., Iwamoto, Y
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Journal Title
J Bone Joint Surg Br 87(12)
Pages: 1643-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Validity of an oblique posterior condylar radiographic view for revision total knee arthroplasty2005
Author(s)
Miura, H., Matsuda, S., Okazaki, K., Kawano, T., Kawamura, Iwamoto, Y
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Journal Title
J Bone Joint Surg Br 87(12)
Pages: 1643-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Anatomical Analysis of the Femoral Condyle in Normal and Osteoarthritic Knees2004
Author(s)
Matsuda, S., Miura, H., Nagamine, R., Mawatari, T., Tokunaga, M., Nabeyama, R., Iwamoto, Y
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Journal Title
J Orthop Res 22
Pages: 104-109
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The oblique posterior condylar radiographic view to evaluate the posterior femoral condyle following total knee arthroplasty2004
Author(s)
Miura, H., Matsuda, S., Mawatari, T., Kawano, T., Nabeyama, R., Iwamoto, Y
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Journal Title
J Bone Joint Surg Am 86
Pages: 47-50
Description
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[Journal Article] The Accuracy of computed tomography-based image-guided total knee arthroplasty2004
Author(s)
Nabeyama, R., Matsuda, S., Miura, H., Mawatari, T., Kawano, T., Iwamoto, Y
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Journal Title
J Bone Joint Surg Br 86
Pages: 366-371
Description
「研究成果報告書概要(欧文)」より
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[Presentation] One-year follow up of osteo-chondral regeneration in rabbit by scaffold free mesenchymal stem cell implantation2007
Author(s)
Nakayama, K., Matsuda, S., Akasaki, Y., Fukagawa, S., Iwamoto, Y
Organizer
7th World Congress of International Cartilage Repair Society
Place of Presentation
Warsaw, Poland
Year and Date
20070928-1002
Description
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[Presentation] Statin induces chondrogenic differentiation in bone marrow stromal cells2007
Author(s)
Akasaki, Y., Matsuda, S., Nakayama, K., Mizu-uchi, H., Fukagawa, S., Iwamoto, Y
Organizer
The 53rd Annual Meeting of Orthopaedic Research Society
Place of Presentation
San Diego, USA
Year and Date
20070211-0214
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Tracking implanted mesenchymal stem cells in vivo with nano-cry stalreagents2005
Author(s)
Nakayama, K., Matsuda, S., Akasaki, Y., Tanaka, K., Miura, H., Iwamoto, Y
Organizer
The 4th Annual Meeting of the Europearn Tissue Engineering Society
Place of Presentation
Munich, Germany
Year and Date
20050831-0903
Description
「研究成果報告書概要(欧文)」より
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[Presentation] In vivo study of scaffold free mesenchymal stem cell plug for cartilage repair2004
Author(s)
Nakayama, K., Matsuda, S., Tanaka, K., Mizuchi, H., Iwamoto, Y
Organizer
The 5th bi-annual meeting of International Cartilage Repair Society
Place of Presentation
Gent, Belgium
Year and Date
20040526-29
Description
「研究成果報告書概要(欧文)」より
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