2005 Fiscal Year Final Research Report Summary
Cartilage regeneration using high performance extra-cellular matrix and development of noninvasive mechanical property evaluation
| Project/Area Number |
16591512
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokai University School of Medicine |
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Principal Investigator |
SATO Masato Tokai Univ., School of Medicine, Assistant Professor, 医学部, 講師 (10056335)
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIDA Joji Tokai Univ., School of Medicine, Professor, 医学部, 教授 (50174347)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Tissue engineering / Extra-cellular matrix / Growth factor / Scaffold / Biomaterial / Heparin / Noninvasive evaluation / Photoacoustic measurement |
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| Research Abstract |
The specific aim of our investigation is to study the potential use of a collagen/heparin-carrying polystyrene (HCPS) composite extracellular matrix for articular cartilage tissue engineering. In this study we created a high- performance extracellular matrix (HpECM) scaffold in order to create an optimal extracellular environment using an HCPS we originally developed, and an atelocollagen honeycomb-shaped scaffold with a membrane seal (ACHMS-scaffold). This scaffold was coated with HCPS to prepare the HpECM-scaffold to enable aggregation of heparin-binding growth factors such as FGF-2 and TGFβwithin the scaffold. Three-dimensional culture of rabbit articular chondrocytes within the HpECM-scaffold and subsequent preparation of a tissue-engineered cartilage were investigated using FGF-2 and TGFβ. The results showed remarkably higher cell proliferative activity within the HpECM-scaffold with FGF-2 and the effect of differentiation induction within the HpECM- scaffold with TGFβ. It was thought that both FGF-2 and TGFβ, which have binding sites to heparin, were stably immobilized in the HpEMC- scaffold since HCPS generated an extracellular environment similar to that of heparan sulfate proteoglycan within the scaffold. Thus, the effects of matricrine and juxtacrine were displayed, and their respective activities were maintained in a long-term effect. At the same time, endogenous growth factor, cytokines, and other functional substances might also be retained within the HpECM-scaffold.
Tissue engineered cartilage using HpECM was evaluated according to the photoacoustic measurement method. As a result, the cartilage increased dynamics strength with the passage of time and HpECM had the tendency to obtain the mechanical characteristic from the early stage of cultivation.
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