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2006 Fiscal Year Final Research Report Summary

Mechanism of permeability increase induced by neuropeptide Y in neurogenic pulmonary edema

Research Project

Project/Area Number 16591532
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionNagoya University

Principal Investigator

NISHIWAKI Kimitoshi  Nagoya University, Graduate School of Medicine, Associate Professor (10189326)

Co-Investigator(Kenkyū-buntansha) ISHIKAWA Naohisa  Aichi Medical University, School of Medicine, Professor (80109321)
Project Period (FY) 2004 – 2006
Keywordsneurogenic pulmonary edema / pulmonary endothelial cell / pulmonary vascular permeability / cell culture / vagal nerve / anesthetic agent
Research Abstract

1. Establishment of neurogenic pulmonary edema induced by fibrin and thrombin injection to the 4th ventricle in rabbit : It was difficult to establish such model and finally abandoned that project.
2. Establishment of partially circulated rabbit lung preparation : This experiment was also stopped, because neurogenic pulmonary edema model in rabbit was failed.
3. Establishment of monolayer cell culture of human pulmonary endothelial cells : establishments of monolayer cell culture were succeeded in not only human pulmonary endothelial cells but also human coronary arterial endothelial cells and human aortic endothelial cells.
4. We investigated the permeability of monolayer of human pulmonary endothelial cells, using monolayer permeability models of endothelial cells, where a double incubation chamber was used for measurement of FITC-labeled albumin transfer through the monolayer. Isoflurane increased the permeability, but sevoflurane and propofol did not. The permeability increase induced by isoflurane was reversed by H-9 (PKG inhibitor), DY9760e (calmodulin inhibitor) and ML-7 (MLCK inhibitor), meaning that isoflurane increased pulmonary endothelial monolayer permeability through cytoskeleton change mediated by cGMP dependent kinase and myosin contraction mediated by myosin light chain kinase.

  • Research Products

    (6 results)

All 2007 2006

All Journal Article (6 results)

  • [Journal Article] Possible role of neuropeptide Y Y3-receptor subtype in rat aortic endothelial cell proliferation under hypoxia,and its specific signal transduction2007

    • Author(s)
      Zhi-Yong Chen
    • Journal Title

      Am J Physiol Heart Circ Physiol 293

      Pages: H959-H967

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Possible role of neuropeptide YY3-receptor subtype in rat aortic endothelial cell proliferation under hypoxia, and its specific signal transduction2007

    • Author(s)
      ZhiYong Chen
    • Journal Title

      Am J Physiol Heart Circ Physiol 293

      Pages: H959-H967

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Different effects of optical isomers of the 5-HT1A receptor antagonist pyrapyridol against postischemic guinea-pig myocardial dysfunction and apoptosis through the mitochondrial permeabiliby transition pore2006

    • Author(s)
      Lei Huang
    • Journal Title

      European Journal of Pharmacology 534

      Pages: 165-177

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Positive inotropic effect of purified green tea catechin derivative in guinea pig hearts;The measurement of celluar Ca2+ and nitric oxide release2006

    • Author(s)
      Yoshihiro Hotta
    • Journal Title

      European Journal of Pharmacology 552

      Pages: 123-130

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Different effects of optical isomers of the 5-HT1A receptor antagonist pyrapyridol against postischeinic guinea-pig myocardial dysfunction and apoptosis through the mitochondrial permeabiliby transition pore.2006

    • Author(s)
      Lei Huang
    • Journal Title

      European Journal of Pharmacology 534

      Pages: 165-177

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Positive inotropic effect of purified green tea catechin derivative in guinea pig hearts ; The measurement of celluar Ca2+ and nitric oxide release.2006

    • Author(s)
      Yoshihiro Hotta
    • Journal Title

      European Journal of Pharmacology 552

      Pages: 123-130

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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